Conditions for transplantation of primordial germ cells in the yellowtail tetra, Astyanax altiparanae

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorChico Mendes Institute of Biodiversity Conservation-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorAcademic Unit-
Autor(es): dc.creatorCoelho, Geovanna Carla Zacheo-
Autor(es): dc.creatorCarvalho, Gabriella Braga-
Autor(es): dc.creatorMonzani, Paulo Sergio-
Autor(es): dc.creatorLopez, Lucia Suarez-
Autor(es): dc.creatorDo Nascimento, Nivaldo Ferreira-
Autor(es): dc.creatorSenhorini, José Augusto-
Autor(es): dc.creatorYasui, George Shigueki-
Data de aceite: dc.date.accessioned2025-08-21T20:58:59Z-
Data de disponibilização: dc.date.available2025-08-21T20:58:59Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2022-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1387/ijdb.230009gc-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306581-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306581-
Descrição: dc.descriptionBiotechniques, including surrogate propagation derived from primordial germ cell (PGC) transplantation, are valuable tools for the reconstitution of endangered fish species. Although promising, there are no previous studies reporting such approaches using neotropical fish species. The aim of this study was to establish germline chimeras in neotropical fish by using the yellowtail tetra Astyanax altiparanae as a model species of the order Characiformes. Germline chimeras were obtained after transplantation of PGCs cultivated under different conditions: saline medium and supplemented with DMEM, amino acids, vitamins, glutamine, pyruvate, and fetal bovine serum, and subsequently transplanted into A. altiparanae triploids and triploid hybrids from the cross between A. altiparanae () and A. fasciatus (). The results indicate ectopic migration in host embryos after transplantation of PGCs cultivated in saline medium. However, PGCs cultivated in supplemented medium migrated to the region of the gonadal ridge in 4.5% of triploid and 19.3% in triploid hybrid. In addition, the higher expression of dnd1, ddx4 and dazl genes was found in PGCs cultivated in supplemented culture medium. This indicates that the culture medium influences the maintenance and development of the cultivated cells. The expression levels of nanos and cxcr4b (related to the differentiation and migration of PGCs) were decreased in PGCs from the supplemented culture medium, supporting the results of ectopic migration. This is the first study to report the transplantation of PGCs to obtain germline chimera in neotropical species. The establishment of micromanipulation procedures in a model neotropical species will open new insights for the conservation of endangered species.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Fish Biotechnology National Center for Research and Conservation of Continental Fish Chico Mendes Institute of Biodiversity Conservation, Pirassununga-
Descrição: dc.descriptionInstitute of Bioscience São Paulo State University-
Descrição: dc.descriptionDepartment of Animal Reproduction School of Veterinary Medicine and Animal Science University of São Paulo-
Descrição: dc.descriptionFederal Rural University of Pernambuco (UFRPE) Academic Unit, Pernambuco State-
Descrição: dc.descriptionInstitute of Bioscience São Paulo State University-
Descrição: dc.descriptionFAPESP: 2010/17429-1-
Descrição: dc.descriptionFAPESP: 2011/11664-1-
Formato: dc.format39-48-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Developmental Biology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbiotechnology-
Palavras-chave: dc.subjectgenebank-
Palavras-chave: dc.subjectgermline chimera-
Palavras-chave: dc.subjectprimordial germ cells-
Palavras-chave: dc.subjectteleost-
Palavras-chave: dc.subjecttransplant-
Título: dc.titleConditions for transplantation of primordial germ cells in the yellowtail tetra, Astyanax altiparanae-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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