Unraveling the impact of melatonin treatment: Oxidative stress, metabolic responses, and morphological changes in HuH7.5 hepatocellular carcinoma cells

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Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorBiological Science Center-
Autor(es): dc.contributorUniversidade Estadual de Maringá (UEM)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Morais, Juliana M.B.-
Autor(es): dc.creatorCruz, Ellen M.S.-
Autor(es): dc.creatorConcato, Virgínia M.-
Autor(es): dc.creatorde Souza, Milena C.-
Autor(es): dc.creatorSantos, Yasmin M.-
Autor(es): dc.creatorQuadreli, Débora H.-
Autor(es): dc.creatorInoue, Fabrício S.R.-
Autor(es): dc.creatorFerreira, Francielle B.-
Autor(es): dc.creatorFernandes, Glaura S.A.-
Autor(es): dc.creatorBidóia, Danielle L.-
Autor(es): dc.creatorMachado, Rayanne R.B.-
Autor(es): dc.creatorChuffa, Luiz Gustavo A.-
Autor(es): dc.creatorPavanelli, Wander R.-
Autor(es): dc.creatorSeiva, Fábio R.F.-
Data de aceite: dc.date.accessioned2025-08-21T19:23:07Z-
Data de disponibilização: dc.date.available2025-08-21T19:23:07Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.prp.2023.155056-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306537-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306537-
Descrição: dc.descriptionIn addition to its highly aggressive nature and late diagnosis, hepatocellular carcinoma (HCC) does not respond effectively to available chemotherapeutic agents. The search is on for an ideal and effective compound with low cost and minimal side effects that can be used as an adjunct to chemotherapeutic regimens. One of the mechanisms involved in the pathology of HCC is the oxidative stress, which plays a critical role in tumor survival and dissemination. Our group has already demonstrated the antitumor potential of melatonin against HuH 7.5 cells. In the present study, we focused on the effects of melatonin on oxidative stress parameters and their consequences on cell metabolism. HuH 7.5 cells were treated with 2 and 4 mM of melatonin for 24 and 48 h. Oxidative stress biomarkers, antioxidant enzyme, mitochondrial membrane potential, formation of lipid bodies and autophagic vacuoles, cell cycle progression, cell death rate and ultrastructural cell alterations were evaluated. The treatment with melatonin increased oxidative stress biomarkers and reduced antioxidant enzyme activities of HuH 7.5 cells. Additionally, melatonin treatment damaged the mitochondrial membrane and increased lipid bodies and autophagic vacuole formation. Melatonin triggered cell cycle arrest and induced cell death by apoptosis. Our results indicate that the treatment of HuH 7.5 cells with melatonin impaired antioxidant defense systems, inhibited cell cycle progression, and caused metabolic stress, culminating in tumor cell death.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionLaboratory of Immunoparasitology of Neglected Diseases and Cancer State University of Londrina (UEL), PR-
Descrição: dc.descriptionNorth of Paraná State University (UENP) Biological Science Center, PR-
Descrição: dc.descriptionGeneral Biology Department Biological Sciences Center State University of Londrina Londrina (UEL), PR-
Descrição: dc.descriptionState University of Maringá (UEM), PR-
Descrição: dc.descriptionDepartment of Structural and Functional Biology São Paulo State University (UNESP) Institute of Bioscience, SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of Bioscience, SP-
Descrição: dc.descriptionDepartment of Structural and Functional Biology São Paulo State University (UNESP) Institute of Bioscience, SP-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences São Paulo State University (UNESP) Institute of Bioscience, SP-
Idioma: dc.languageen-
Relação: dc.relationPathology Research and Practice-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdjuvant therapy-
Palavras-chave: dc.subjectLiver cancer-
Palavras-chave: dc.subjectMelatonin-
Palavras-chave: dc.subjectOxidative stress-
Título: dc.titleUnraveling the impact of melatonin treatment: Oxidative stress, metabolic responses, and morphological changes in HuH7.5 hepatocellular carcinoma cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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