Transcription factors and miRNA act as contrary regulators of gene expression in the testis and epididymis of Bos indicus animals

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorThe University of Queensland-
Autor(es): dc.contributorUniversity of Washington-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAfonso, Juliana-
Autor(es): dc.creatorLima, Andressa Oliveira-
Autor(es): dc.creatorde Sousa, Marco Antonio Perpétuo-
Autor(es): dc.creatorde Athayde, Flávia Regina Florêncio-
Autor(es): dc.creatorFortes, Marina Rufino Salinas-
Data de aceite: dc.date.accessioned2025-08-21T23:18:10Z-
Data de disponibilização: dc.date.available2025-08-21T23:18:10Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-03-20-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.gene.2024.148133-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306431-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306431-
Descrição: dc.descriptionSpermatogenesis is highly conserved among mammalians, but its gene expression and regulatory profile are not entirely known. As transcription factors (TFs) and miRNAs are crucial for gene expression regulation, identifying genes negatively regulated by miRNAs and positively regulated by TFs in the testis and epididymis can provide a deeper understanding of gene expression and regulatory patterns. To do this, we used expression data coming from RNA-Seq and miRNA-Seq experiments made with biopsies from testicular parenchyma, head of the epididymis, and tail of the epididymis of four Brahman bulls. We identified miRNA differentially expressed (DE) by comparing the three distinct tissues. A co-expression analysis combined with a regulatory impact factor approach identified miRNAs and TFs with regulatory impact over gene expression regulation in the Bos indicus tissues studied. We identified 116 DE miRNAs, 206 miRNAs and 237 TFs with a significant regulatory impact on mRNA patterns in the tissues’ comparisons. bta-miR-196b was the only DE miRNA for all tissue comparisons and it may be a regulator of spermatogenesis through its links with adipogenesis and insulin biosynthesis. DE genes and TFs involved in contrary regulations between the epididymis head and testis parenchyma were associated with spermatogenesis, sexual reproduction, and sperm motility. Our results provide possible mechanisms, governed by the contrary effect of miRNA and TF, leading to the differential expression between the studied tissues. We have demonstrated that our predictions of miRNAs and TFs co-regulations over target DE genes can retrieve known regulatory mechanisms and predict new ones that merit further validation.-
Descrição: dc.descriptionEmbrapa Pecuária Sudeste, São Paulo-
Descrição: dc.descriptionSchool of Chemistry and Molecular Biosciences The University of Queensland-
Descrição: dc.descriptionDivision of Medical Genetics Department of Genome Sciences Department of Medicine University of Washington-
Descrição: dc.descriptionDepartamento de Produção e Saúde Animal Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Medicina Veterinária-
Descrição: dc.descriptionDepartamento de Produção e Saúde Animal Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP) Faculdade de Medicina Veterinária-
Idioma: dc.languageen-
Relação: dc.relationGene-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBrahman-
Palavras-chave: dc.subjectCoexpression-
Palavras-chave: dc.subjectDifferential expression-
Palavras-chave: dc.subjectReproduction-
Palavras-chave: dc.subjectSpermatogenesis-
Título: dc.titleTranscription factors and miRNA act as contrary regulators of gene expression in the testis and epididymis of Bos indicus animals-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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