Functional polymorphisms of NOS3 and GUCY1A3 affect both nitric oxide formation and association with hypertensive disorders of pregnancy

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de Minas Gerais (UFMG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Mississippi Medical Center-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.creatorPereira, Daniela A.-
Autor(es): dc.creatorLuizon, Marcelo R.-
Autor(es): dc.creatorPalei, Ana C.-
Autor(es): dc.creatorTanus-Santos, José E.-
Autor(es): dc.creatorCavalli, Ricardo C.-
Autor(es): dc.creatorSandrim, Valeria C.-
Data de aceite: dc.date.accessioned2025-08-21T20:09:59Z-
Data de disponibilização: dc.date.available2025-08-21T20:09:59Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-02-26-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3389/fgene.2024.1293082-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306412-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306412-
Descrição: dc.descriptionImpaired nitric oxide (NO) formation may be associated with endothelial dysfunction and increased cardiovascular disease risk in preeclampsia (PE). Functional single-nucleotide polymorphisms (SNPs) of nitric oxide synthase 3 (NOS3) (rs3918226) and guanylate cyclase 1, soluble, alpha 3 (GUCY1A3) (rs7692387) increase susceptibility to the adverse consequences due to inadequate generation of NO by the endothelium. However, no previous study has examined whether these SNPs affect NO formation in healthy pregnancy and in gestational hypertension (GH) and PE. Here, we compared the alleles and genotypes of NOS3 (rs3918226) and GUCY1A3 (rs7692387) SNPs in normotensive pregnant women (NP, n = 153), in GH (n = 96) and PE (n = 163), and examined whether these SNPs affect plasma nitrite concentrations (a marker of NO formation) in these groups. We further examined whether the interaction among SNP genotypes is associated with GH and PE. Genotypes were determined using TaqMan allele discrimination assays, and plasma nitrite concentrations were determined by an ozone-based chemiluminescence assay. Multifactor dimensionality reduction was used to examine the interactions among SNP genotypes. Regarding NOS3 rs3918226, the CT genotype (p = 0.046) and T allele (p = 0.020) were more frequent in NP than in GH, and GH patients carrying the CT+TT genotypes showed lower nitrite concentrations than NP carrying the CT+TT genotypes (p < 0.05). Regarding GUCY1A3 rs7692387, the GA genotype (p = 0.013) and A allele (p = 0.016) were more frequent in PE than in NP, and NP women carrying the GG genotype showed higher nitrite concentrations than GH or PE patients carrying the GG genotype (p < 0.05). However, we found no significant interactions among genotypes for these functional SNPs to be associated with GH or PE. Our novel findings suggest that NOS3 rs3918226 and GUCY1A3 rs7692387 may affect NO formation and association with hypertensive disorders of pregnancy.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionCenter for Information Technology-
Descrição: dc.descriptionCenter for Scientific Review-
Descrição: dc.descriptionNational Institutes of Health-
Descrição: dc.descriptionOffice of Extramural Research, National Institutes of Health-
Descrição: dc.descriptionOffice of Research Infrastructure Programs, National Institutes of Health-
Descrição: dc.descriptionDepartment of Genetics Ecology and Evolution Institute of Biological Sciences Federal University of Minas Gerais, Minas Gerais-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionDepartment of Surgery University of Mississippi Medical Center-
Descrição: dc.descriptionDepartment of Pharmacology Ribeirao Preto Medical School University of Sao Paulo-
Descrição: dc.descriptionDepartment of Gynecology and Obstetrics Ribeirao Preto Medical School University of Sao Paulo-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences Universidade Estadual Paulista (UNESP)-
Descrição: dc.descriptionFAPESP: 2019/07230-8 2021/12010-7-
Descrição: dc.descriptionCNPq: 314486/2023-2 308504/2021-6-
Descrição: dc.descriptionCAPES: Finance Code 001-
Descrição: dc.descriptionCenter for Information Technology: K01HL159032 R01HL148191 U54GM115428-
Descrição: dc.descriptionCenter for Scientific Review: K01HL159032 R01HL148191 U54GM115428-
Descrição: dc.descriptionNational Institutes of Health: K01HL159032 R01HL148191 U54GM115428-
Descrição: dc.descriptionOffice of Extramural Research, National Institutes of Health: K01HL159032 R01HL148191 U54GM115428-
Descrição: dc.descriptionOffice of Research Infrastructure Programs, National Institutes of Health: K01HL159032 R01HL148191 U54GM115428-
Idioma: dc.languageen-
Relação: dc.relationFrontiers in Genetics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectgenetic polymorphisms-
Palavras-chave: dc.subjectgestational hypertension-
Palavras-chave: dc.subjectguanylate cyclase 1 soluble alpha 3-
Palavras-chave: dc.subjectnitric oxide-
Palavras-chave: dc.subjectnitric oxide synthase 3-
Palavras-chave: dc.subjectnitrite-
Palavras-chave: dc.subjectpreeclampsia-
Palavras-chave: dc.subjectpregnancy-
Título: dc.titleFunctional polymorphisms of NOS3 and GUCY1A3 affect both nitric oxide formation and association with hypertensive disorders of pregnancy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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