Preparative Fractionation of Brazilian Red Propolis Extract Using Step-Gradient Counter-Current Chromatography

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Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorBrazilian Center for Research in Energy and Materials (CNPEM)-
Autor(es): dc.contributorMackenzie Presbyterian University-
Autor(es): dc.contributorBrunel University London-
Autor(es): dc.creatorLopez, Begoña Gimenez-Cassina-
Autor(es): dc.creatorMarcucci, Maria Cristina-
Autor(es): dc.creatorRocco, Silvana Aparecida-
Autor(es): dc.creatorSforça, Maurício Luís-
Autor(es): dc.creatorEberlin, Marcos Nogueira-
Autor(es): dc.creatorHewitson, Peter-
Autor(es): dc.creatorIgnatova, Svetlana-
Autor(es): dc.creatorSawaya, Alexandra Christine Helena Frankland-
Data de aceite: dc.date.accessioned2025-08-21T18:50:45Z-
Data de disponibilização: dc.date.available2025-08-21T18:50:45Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/molecules29122757-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306386-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306386-
Descrição: dc.descriptionPropolis is a resinous bee product with a very complex composition, which is dependent upon the plant sources that bees visit. Due to the promising antimicrobial activities of red Brazilian propolis, it is paramount to identify the compounds responsible for it, which, in most of the cases, are not commercially available. The aim of this study was to develop a quick and clean preparative-scale methodology for preparing fractions of red propolis directly from a complex crude ethanol extract by combining the extractive capacity of counter-current chromatography (CCC) with preparative HPLC. The CCC method development included step gradient elution for the removal of waxes (which can bind to and block HPLC columns), sample injection in a single solvent to improve stationary phase stability, and a change in the mobile phase flow pattern, resulting in the loading of 2.5 g of the Brazilian red propolis crude extract on a 912.5 mL Midi CCC column. Three compounds were subsequently isolated from the concentrated fractions by preparative HPLC and identified by NMR and high-resolution MS: red pigment, retusapurpurin A; the isoflavan 3(R)-7-O-methylvestitol; and the prenylated benzophenone isomers xanthochymol/isoxanthochymol. These compounds are markers of red propolis that contribute to its therapeutic properties, and the amount isolated allows for further biological activities testing and for their use as chromatographic standards.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionFaculty of Pharmaceutical Science State University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SP-
Descrição: dc.descriptionBrazilian Biosciences National Laboratory (LNBio) Brazilian Center for Research in Energy and Materials (CNPEM), SP-
Descrição: dc.descriptionMACKGRAPHE—School of Engineering Mackenzie Presbyterian University, SP-
Descrição: dc.descriptionDepartment of Chemical Engineering College of Engineering Design and Physical Sciences (CEDPS) Brunel University London-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2013/20313-3-
Descrição: dc.descriptionCNPq: 306100/2021-5-
Idioma: dc.languageen-
Relação: dc.relationMolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3(R)-7-O-methylvestitol-
Palavras-chave: dc.subjectinjection strategy-
Palavras-chave: dc.subjectprenylated benzophenone isomers-
Palavras-chave: dc.subjectpreparative fractionation-
Palavras-chave: dc.subjectretusapurpurin A-
Título: dc.titlePreparative Fractionation of Brazilian Red Propolis Extract Using Step-Gradient Counter-Current Chromatography-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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