Molecular detection of Paracoccidioides spp. in environmental samples from the Southwestern Amazon

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorFederal University of Acre-
Autor(es): dc.contributorUniversity of Brasília-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorFerreira, Iasminy R. Silva-
Autor(es): dc.creatorde M. Teixeira, Marcus-
Autor(es): dc.creatorde Araújo, Atilon V.-
Autor(es): dc.creatorBagagli, Eduardo-
Autor(es): dc.creatorGarces, Hans G.-
Autor(es): dc.creatorPeters, Leila P.-
Autor(es): dc.creatorCarvalho, Clarice M.-
Data de aceite: dc.date.accessioned2025-08-21T16:02:53Z-
Data de disponibilização: dc.date.available2025-08-21T16:02:53Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s42770-024-01256-7-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306238-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306238-
Descrição: dc.descriptionParacoccidioidomycosis is an infection with the potential for environmental dissemination, especially in regions of hot and humid climate, where human cases have been recorded in the Southwestern Amazon of Brazil, specifically in the state of Acre. Despite studies providing information about the presence of these fungi in soil and animal samples, such as armadillos, further investigations are still needed to determine the epidemiological distribution of the genus Paracoccidioides. The aim of this study was to detect the occurrence of Paracoccidioides fungi in the Southwestern Amazon. To achieve this, 60 soil samples were collected from armadillo burrows on rural properties in the in the municipalities of Acrelândia, Bujari, Plácido de Castro, Rio Branco, Sena Madureira, and Senador Guiomard, located in the state of Acre, Brazil. Fungal DNA was extracted from these samples using the DNEASY® PowerSoil kit—Quiagen, followed by Nested PCR technique with ITS4 and ITS5 as external primers, and PBITS-E and PBITS-R as internal primers. DNA amplification products of about 380 bp compatible with Paracoccidioides spp. were detected in six samples (10%), being sequenced and identified as P. brasiliensis. These findings indicate that the soils of the Acre state could be considered a potential source for Paracoccidioides spp., suggesting that local infections are likely.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionGraduate Program in Biodiversity and Biotechnology Federal University of Acre, AC-
Descrição: dc.descriptionTropical Medicine Center Faculty of Medicine University of Brasília, Campus Universitário Darcy Ribeiro, DF-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences São Paulo State University “Júlio de Mesquita Filho”, SP-
Descrição: dc.descriptionCenter for Biological and Natural Sciences Federal University of Acre, AC-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences São Paulo State University “Júlio de Mesquita Filho”, SP-
Descrição: dc.descriptionCAPES: 0889/2018-
Formato: dc.format837-842-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Journal of Microbiology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcre-
Palavras-chave: dc.subjectArmadillo-
Palavras-chave: dc.subjectBrazil-
Palavras-chave: dc.subjectP.brasiliensis-
Palavras-chave: dc.subjectParacoccidioidomycosis-
Título: dc.titleMolecular detection of Paracoccidioides spp. in environmental samples from the Southwestern Amazon-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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