Potential role of inoculum size in biomass accumulation and antioxidant secondary metabolites production in adventitious root cultures of Stevia rebaudiana

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorThe University of Agriculture-
Autor(es): dc.contributorUniversity of Swat-
Autor(es): dc.contributorKing Saud University-
Autor(es): dc.contributorUniversity of Antwerp-
Autor(es): dc.contributorJiangsu University-
Autor(es): dc.contributorKing Abdullah University of Science and Technology (KAUST)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAhmad, Naveed-
Autor(es): dc.creatorAhmad, Nisar-
Autor(es): dc.creatorSajid, Muhammad-
Autor(es): dc.creatorRauf, Kamran-
Autor(es): dc.creatorIqbal, Mudassar-
Autor(es): dc.creatorNuman, Muhammad-
Autor(es): dc.creatorUllah, Irfan-
Autor(es): dc.creatorKhan, Amir-
Autor(es): dc.creatorAl-Qahtani, Wahidah H.-
Autor(es): dc.creatorAbdel-Maksoud, Mostafa A.-
Autor(es): dc.creatorAbdElgawad, Hamada-
Autor(es): dc.creatorIqbal, Babar-
Autor(es): dc.creatorJalal, Arshad-
Data de aceite: dc.date.accessioned2025-08-21T15:35:39Z-
Data de disponibilização: dc.date.available2025-08-21T15:35:39Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1080/11263504.2025.2460459-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306101-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306101-
Descrição: dc.descriptionThe present study aimed to investigate the effect of initial inoculum size on adventitious root growth and secondary metabolite production in Stevia rebaudiana s root cultures, using various initial inoculum sizes (0.5, 1.0, 1.5, and 2.0 g). The roots were collected from the in-vitro seed-derived plantlets and transferred to a half-strength MS medium with 0.5 mg l−1 naphthalene acetic acid (NAA) to establish adventitious root cultures. Growth kinetics and fresh and dry biomass of adventitious root cultures were enhanced with inoculum size from 0.5–2.0 g. Adventitious root cultures did not show lag phases however, the growth curve was increased at an early stage (day 3) of log phases and sustained for 27 days of culture. The fresh and dry biomass accumulations of the adventitious root cultures were increased by 51 and 120% with 1.5 g inoculum size as compared to a smaller inoculum size (0.5 g). Adventitious root cultures at an initial inoculum size of 2.0 g had a significantly higher content of total phenolics (TPC; 41.46 mg g−1 DW), total flavonoids (TFC; 33.44 mg g−1 DW), and around 98.82% higher potential for scavenging free radicals. In addition, the initial inoculum size of 1.5 g was observed with higher dulcoside contents (0.71 mg g−1 DW), and an inoculum size of 1.0 g was noted with higher content of stevioside (64.75 mg g−1 DW) and rebaudioside (29.67 mg g−1 DW). Therefore, it is concluded that adventitious root cultures accumulated greater fresh and dry biomasses at an inoculum size of 1.5 g, a higher amount of TPC, TFC, and DRSA at 2.0 g, and stevioside and rebaudioside contents at 1.0 g.-
Descrição: dc.descriptionDepartment of Horticulture The University of Agriculture, Peshawar-
Descrição: dc.descriptionCentre for Biotechnology and Microbiology University of Swat-
Descrição: dc.descriptionDepartment of Agricultural Chemistry The University of Agriculture-
Descrição: dc.descriptionDepartment of Food Sciences & Nutrition College of Food and Agricultural Sciences King Saud University-
Descrição: dc.descriptionBotany and Microbiology department college of science King Saud University-
Descrição: dc.descriptionIntegrated Molecular Plant Physiology Research Department of Biology University of Antwerp-
Descrição: dc.descriptionSchool of Environment and Safety Engineering School of Emergency Management Jiangsu University-
Descrição: dc.descriptionThe BioActives Lab Center of Excellence for Sustainable Food Security Division of Biological and Environmental Sciences King Abdullah University of Science and Technology (KAUST)-
Descrição: dc.descriptionSchool of Engineering Department of Plant Health Soil and Rural Engineering Sao Paulo State University-
Descrição: dc.descriptionSchool of Engineering Department of Plant Health Soil and Rural Engineering Sao Paulo State University-
Idioma: dc.languageen-
Relação: dc.relationPlant Biosystems-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAdventitious root-
Palavras-chave: dc.subjectanioxidant-
Palavras-chave: dc.subjectinoculum-
Palavras-chave: dc.subjectrebaudioside-
Palavras-chave: dc.subjectStevia rebaudiana-
Palavras-chave: dc.subjectstevioside-
Título: dc.titlePotential role of inoculum size in biomass accumulation and antioxidant secondary metabolites production in adventitious root cultures of Stevia rebaudiana-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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