K-aurein: A notable aurein 1.2-derived peptide that modulates Candida albicans filamentation and reduces biofilm biomass

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Autor(es): dc.contributorFederal University of Ceara—UFC-
Autor(es): dc.contributorUniversity Estate of Vale do Acaraú UVA-
Autor(es): dc.contributorFederal University of Jatai-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity Center INTA—UNINTA-
Autor(es): dc.creatorSilva, Maria Laína-
Autor(es): dc.creatorCarneiro, Maria Nágila-
Autor(es): dc.creatorCavalcante, Rafaela Mesquita Bastos-
Autor(es): dc.creatorGuerrero, Jesús Alberto Pérez-
Autor(es): dc.creatorFontenelle, Raquel Oliveira Santos-
Autor(es): dc.creatorLorenzón, Esteban Nicolás-
Autor(es): dc.creatorCilli, Eduardo Maffud-
Autor(es): dc.creatorCarneiro, Victor Alves-
Data de aceite: dc.date.accessioned2025-08-21T21:37:30Z-
Data de disponibilização: dc.date.available2025-08-21T21:37:30Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00726-023-03288-z-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306068-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306068-
Descrição: dc.descriptionCandida albicans is considered one of the most important opportunistic fungi due to the large arsenal of virulence factors that help throughout the progress of the infection. In this sense, antimicrobial peptides (AMPs) appear as an alternative, with great antifungal action. Among these, aurein 1.2 has been widely explored, becoming the basis for the discovery of new AMPs, such as K-aurein (K-au). Thus, this study evaluated the anti-C. albicans potential of K-au against virulence factors, planktonic growth, and biofilm formation of clinical isolates. Firstly, K-au antifungal activity was determined by the microdilution method and time-kill curve. The inhibition of hydrolytic enzyme secretion (proteinase, phospholipase, and hemolysin) and germ tube formation was tested. Then, the antibiofilm potential of K-au was verified through biomass quantification and scanning electron microscopy (SEM). All tests were compared with the classical antifungal drug, amphotericin B (AmB). The outcomes showed fungicidal action of K-au at 62.50 µg mL−1 for all isolates, with a time of action around 150–180 min, determined by the time-kill curve. K-au-treated cells decreased by around 40% of the germinative tube compared to the control. Additionally, K-au inhibited the biofilm formation by more than 90% compared to AmB and the control group. SEM images show apparent cellular disaggregation without the formation of filamentous structures. Therefore, the findings suggest a promising anti-C. albicans effect of K-au due to its fungicidal activity against planktonic cells, or its ability to inhibit important virulence factors like germ tube and biofilm formation. Thus, this peptide could be explored as a useful compound against C. albicans-related infection.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Biofilms and Antimicrobial Agents (LaBAM) Faculty of Medicine Federal University of Ceara—UFC-
Descrição: dc.descriptionLaboratory of Microbiology (LABMIC) University Estate of Vale do Acaraú UVA-
Descrição: dc.descriptionHealth Sciences Academic Unit Federal University of Jatai-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Estadual University of São Paulo—UNESP-
Descrição: dc.descriptionCenter for Bioprospecting and Applied Molecular Experimentation (NUBEM) University Center INTA—UNINTA-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Estadual University of São Paulo—UNESP-
Descrição: dc.descriptionFAPESP: #2013/07600-3-
Formato: dc.format1003-1012-
Idioma: dc.languageen-
Relação: dc.relationAmino Acids-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial peptide-
Palavras-chave: dc.subjectAurein 1.2-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectCandida-
Palavras-chave: dc.subjectVirulence-
Título: dc.titleK-aurein: A notable aurein 1.2-derived peptide that modulates Candida albicans filamentation and reduces biofilm biomass-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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