CRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole (Cynoglossus semilaevis) Testis Cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorShanghai Ocean University-
Autor(es): dc.contributorChinese Academy of Fishery Sciences-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorMinistry of Education-
Autor(es): dc.contributorQingdao Marine Science and Technology Center-
Autor(es): dc.creatorSun, Yanxu-
Autor(es): dc.creatorWang, Hong-Yan-
Autor(es): dc.creatorLiu, Binghua-
Autor(es): dc.creatorYue, Bowen-
Autor(es): dc.creatorLiu, Qian-
Autor(es): dc.creatorLiu, Yuyan-
Autor(es): dc.creatorRosa, Ivana F.-
Autor(es): dc.creatorDoretto, Lucas B.-
Autor(es): dc.creatorHan, Shenglei-
Autor(es): dc.creatorLin, Lei-
Autor(es): dc.creatorGong, Xiaoling-
Autor(es): dc.creatorShao, Changwei-
Data de aceite: dc.date.accessioned2025-08-21T22:24:59Z-
Data de disponibilização: dc.date.available2025-08-21T22:24:59Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ijms25147637-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/306012-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/306012-
Descrição: dc.descriptionDNA methylation is a key epigenetic mechanism orchestrating gene expression networks in many biological processes. Nonetheless, studying the role of specific gene methylation events in fish faces challenges. In this study, we validate the regulation of DNA methylation on empty spiracles homeobox 2 (emx2) expression with decitabine treatment in Chinese tongue sole testis cells. We used the emx2 gene as the target gene and developed a new DNA methylation editing system by fusing dnmt3a with catalytic dead Cas9 (dCas9) and demonstrated its ability for sequence-specific DNA methylation editing. Results revealed that utilizing dCas9-dnmt3a to target emx2 promoter region led to increased DNA methylation levels and decreased emx2 expression in Chinese tongue sole testis cells. More importantly, the DNA methylation editing significantly suppressed the expression of MYC proto-oncogene, bHLH transcription factor (myc), one target gene of emx2. Furthermore, we assessed the off-target effects of dCas9-dnmt3a and confirmed no significant impact on the predicted off-target gene expression. Taken together, we developed the first DNA methylation editing system in marine species and demonstrated its effective editing ability in Chinese tongue sole cells. This provides a new strategy for both epigenetic research and molecular breeding of marine species.-
Descrição: dc.descriptionNational Key Research and Development Program of China-
Descrição: dc.descriptionNational Natural Science Foundation of China-
Descrição: dc.descriptionCollege of Fisheries and Life Science Shanghai Ocean University-
Descrição: dc.descriptionState Key Laboratory of Mariculture Biobreeding and Sustainable Goods Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionKey Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University) Ministry of Education-
Descrição: dc.descriptionNational Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean University-
Descrição: dc.descriptionLaboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)-
Descrição: dc.descriptionNational Key Research and Development Program of China: 2022YFD2400100-
Descrição: dc.descriptionNational Natural Science Foundation of China: 32002371-
Idioma: dc.languageen-
Relação: dc.relationInternational Journal of Molecular Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCRISPR/dCas9-
Palavras-chave: dc.subjectCynoglossus semilaevis-
Palavras-chave: dc.subjectDNA methylation-
Palavras-chave: dc.subjectemx2-
Título: dc.titleCRISPR/dCas9-Mediated DNA Methylation Editing on emx2 in Chinese Tongue Sole (Cynoglossus semilaevis) Testis Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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