Acquired enamel pellicle and biofilm engineering with a combination of acid-resistant proteins (CaneCPI-5, StN15, and Hemoglobin) for enhanced protection against dental caries - in vivo and in vitro investigations

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSaarland University-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorAraujo, Tamara Teodoro-
Autor(es): dc.creatorDionizio, Aline-
Autor(es): dc.creatorCarvalho, Thamyris Souza-
Autor(es): dc.creatorBoas Feitosa, Chelsea Maria Vilas-
Autor(es): dc.creatorVertuan, Mariele-
Autor(es): dc.creatorCâmara, João Victor Frazão-
Autor(es): dc.creatorHenrique-Silva, Flavio-
Autor(es): dc.creatorMarchetto, Reinaldo-
Autor(es): dc.creatorChiaratti, Marcos Roberto-
Autor(es): dc.creatorSantos, Angélica Camargo-
Autor(es): dc.creatorAlves, Lindomar Oliveira-
Autor(es): dc.creatorFerro, Milene-
Autor(es): dc.creatorBuzalaf, Marília Afonso Rabelo-
Data de aceite: dc.date.accessioned2025-08-21T16:27:53Z-
Data de disponibilização: dc.date.available2025-08-21T16:27:53Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s00784-024-05651-0-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305833-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305833-
Descrição: dc.descriptionObjective: This study was designed in two-legs. In the in vivo, we explored the potential of a rinse solution containing a combination (Comb) of 0.1 mg/mL CaneCPI-5 (sugarcane-derive cystatin), 1.88 × 10− 5M StN15 (statherin-derived peptide) and 1.0 mg/mL hemoglobin (Hb) to change the protein profile of the acquired enamel pellicle(AEP) and the microbiome of the enamel biofilm. The in vitro, was designed to reveal the effects of Comb on the viability and bacterial composition of the microcosm biofilm, as well as on enamel demineralization. Materials and methods: In vivo study, 10 participants rinsed (10mL,1 min) with either deionized water (H2O-control) or Comb. AEP and biofilm were collected after 2 and 3 h, respectively, after rinsing. AEP samples underwent proteomics analysis, while biofilm microbiome was assessed via 16 S-rRNA Next Generation Sequencing(NGS). In vitro study, a microcosm biofilm protocol was employed. Ninety-six enamel specimens were treated with: 1)Phosphate-Buffered Solution-PBS(negative-control), 2)0.12%Chlorhexidine, 3)500ppmNaF and 4)Comb. Resazurin, colony-forming-units(CFU) and Transversal Microradiography(TMR) were performed. Results: The proteomic results revealed higher quantity of proteins in the Comb compared to control associated with immune system response and oral microbial adhesion. Microbiome showed a significant increase in bacteria linked to a healthy microbiota, in the Comb group. In the in vitro study, Comb group was only efficient in reducing mineral-loss and lesion-depth compared to the PBS. Conclusions: The AEP modification altered the subsequent layers, affecting the initial process of bacterial adhesion of pathogenic and commensal bacteria, as well as enamel demineralization. Clinical relevance: Comb group shows promise in shaping oral health by potentially introducing innovative approaches to prevent enamel demineralization and deter tooth decay.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, Bauru-
Descrição: dc.descriptionClinic of Operative Dentistry Periodontology and Preventive Dentistry University Hospital Saarland University, Building 73, Saarland-
Descrição: dc.descriptionDepartamento de Genética e Evolução Universidade Federal de São Carlos-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Paulista State University (UNESP)-
Descrição: dc.descriptionDepartment of General and Applied Biology Paulista State University (UNESP)-
Descrição: dc.descriptionDepartment of Biochemistry and Organic Chemistry Paulista State University (UNESP)-
Descrição: dc.descriptionDepartment of General and Applied Biology Paulista State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2019/08032-5-
Descrição: dc.descriptionFAPESP: 2019/26070-1-
Descrição: dc.descriptionCNPq: 302371/2018–4 ; 304554/2023-5-
Idioma: dc.languageen-
Relação: dc.relationClinical Oral Investigations-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAcquired enamel pellicle-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectCaneCPI-5-
Palavras-chave: dc.subjectDental caries-
Palavras-chave: dc.subjectHemoglobin-
Palavras-chave: dc.subjectStatherin-
Título: dc.titleAcquired enamel pellicle and biofilm engineering with a combination of acid-resistant proteins (CaneCPI-5, StN15, and Hemoglobin) for enhanced protection against dental caries - in vivo and in vitro investigations-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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