Polymeric Nanocapsules Loaded with Lidocaine: A Promising Formulation for Topical Dental Anesthesia

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorGuarulhos University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorda Silva, Camila Batista-
Autor(es): dc.creatordos Santos, Cleiton Pita-
Autor(es): dc.creatorSerpe, Luciano-
Autor(es): dc.creatorSanchez, Jonny Burga-
Autor(es): dc.creatorFerreira, Luiz Eduardo Nunes-
Autor(es): dc.creatorde Melo, Nathalie Ferreira Silva-
Autor(es): dc.creatorGroppo, Francisco Carlos-
Autor(es): dc.creatorFraceto, Leonardo Fernandes-
Autor(es): dc.creatorVolpato, Maria Cristina-
Autor(es): dc.creatorFranz-Montan, Michelle-
Data de aceite: dc.date.accessioned2025-08-21T20:34:28Z-
Data de disponibilização: dc.date.available2025-08-21T20:34:28Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ph17040485-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305722-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305722-
Descrição: dc.descriptionLidocaine is the most commonly used local anesthetic worldwide, known for its rapid onset and moderate duration of anesthesia. However, it is short-lived and does not effectively promote effective topical anesthesia in the oral cavity when used alone. Our aim was to investigate whether an approximate 50% encapsulation of lidocaine in poly(ε-caprolactone) nanocapsules (LDC-Nano) would be able to increase its permeation and analgesic efficacy and reduce cytotoxicity. In this study, we characterized LDC-Nano and conducted MTT tests with HaCaT cells to assess their in vitro cytotoxicity. Additionally, in vitro permeation assays across the pig esophageal epithelium and the anesthetic efficacy of the hind paw incision model in rats were performed. Plain lidocaine (LDC) was compared with LDC-Nano and lidocaine hydrochloride plus epinephrine (LDC-Epi). The physicochemical characteristics of LDC-Nano were satisfactory (pH: 8.1 ± 0.21; polydispersity index: 0.08 ± 0.01; mean diameter (nm): 557.8 ± 22.7; and encapsulation efficiency (%): 51.8 ± 1.87) and remained stable for up to 4 months. LDC-Nano presented similar in vitro cytotoxicity to LDC but was higher than LDC-Epi (LD50: LDC = 0.48%; LDC-Nano = 0.47%; and LDC-Epi = 0.58%; p < 0.0001). Encapsulation increased the permeability coefficient about 6.6 times and about 7.5 the steady-state flux of lidocaine across the mucosal epithelium. Both encapsulation and epinephrine improved anesthesia duration, with epinephrine demonstrating superior efficacy (100% of animals were anesthetized up to 100, 30, and 20 min when LDC-Epi, LDC-nano, and LDC were used, respectively). Although LDC-Epi demonstrated superior in vivo anesthetic efficacy, the in vitro permeation and cytotoxicity of LDC-Nano indicate promising avenues for future research, particularly in exploring its potential application as a topical anesthetic in the oral cavity.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Biosciences Piracicaba Dental School Universidade Estadual de Campinas, Av. Limeira, 901, SP-
Descrição: dc.descriptionLaboratory of Inflammation and Immunology Guarulhos University, SP-
Descrição: dc.descriptionDepartment of Environmental Engineering São Paulo State University, SP-
Descrição: dc.descriptionDepartment of Environmental Engineering São Paulo State University, SP-
Descrição: dc.descriptionFAPESP: #2012/02492-5-
Descrição: dc.descriptionFAPESP: #2012/02539-1-
Descrição: dc.descriptionFAPESP: #2012/02590-7-
Idioma: dc.languageen-
Relação: dc.relationPharmaceuticals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectanesthetic efficacy-
Palavras-chave: dc.subjectcytotoxicity-
Palavras-chave: dc.subjectdentistry-
Palavras-chave: dc.subjectlidocaine-
Palavras-chave: dc.subjectpermeation-
Palavras-chave: dc.subjectpoly(epsilon-caprolactone) nanocapsules-
Palavras-chave: dc.subjecttopical anesthesia-
Título: dc.titlePolymeric Nanocapsules Loaded with Lidocaine: A Promising Formulation for Topical Dental Anesthesia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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