Clinical Implications of Immune Checkpoints and the RANK/RANK-L Signaling Pathway in High-Grade Canine Mast Cell Tumors

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorTalavera Guillén, Noelia C.-
Autor(es): dc.creatorBarboza de Nardi, Andrigo-
Autor(es): dc.creatorNoleto de Paiva, Felipe-
Autor(es): dc.creatorDias, Queila Cristina-
Autor(es): dc.creatorPinheiro Fantinatti, Alexandra-
Autor(es): dc.creatorFávaro, Wagner José-
Data de aceite: dc.date.accessioned2025-08-21T18:44:36Z-
Data de disponibilização: dc.date.available2025-08-21T18:44:36Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ani13121888-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305699-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305699-
Descrição: dc.descriptionMast cell tumors (MCTs) are the most common malignant cutaneous tumors in dogs, and they present extremely variable biological behavior. The interaction between RANK, RANK-L, and immune checkpoints is frequently detected in the tumor microenvironment, and, together, they participate in every stage of cancer development. Thus, the aim of this study was to characterize the molecular profiles of PD-L1, CTLA-4, RANK/RANK-L signaling pathway, and IFN-γ in primary tumors and lymph node metastases. Formalin-fixed, paraffin-embedded slides of MCTs and metastatic lymph nodes of ten dogs were submitted to immunohistochemical investigations. The results demonstrated that the tumor microenvironment of the high-grade mast cell tumors showed moderate or intense immunolabeling of all proteins, and the lymph node metastases also showed moderate or intense immunolabeling of checkpoint proteins. In addition, MCTs larger than 3 cm were associated with intensified PD-L1 (p = 0.03) in metastatic lymph nodes and RANK-L (p = 0.049) immunoreactivity in the tumor. Furthermore, dogs with a survival time of less than 6 months showed higher PD-L1 immunoreactivity (p = 0.042). In conclusion, high-grade MCT is associated with an immunosuppressive microenvironment that exhibits elevated RANK/RANK-L signaling and enhanced immune checkpoint immunoreactivity, potentially facilitating intratumorally immune escape. These biomarkers show promise as clinical indicators of disease progression and might response to immunotherapy in dogs with high-grade MCTs, thus emphasizing their importance for guiding treatment decisions and improving outcomes.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Veterinary Clinics and Surgery São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Structural and Functional Biology University of Campinas (UNICAMP)-
Descrição: dc.descriptionDepartment of Veterinary Clinics and Surgery São Paulo State University (UNESP)-
Idioma: dc.languageen-
Relação: dc.relationAnimals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectdogs-
Palavras-chave: dc.subjectimmune checkpoints-
Palavras-chave: dc.subjectimmunotherapy-
Palavras-chave: dc.subjectmast cell tumor-
Palavras-chave: dc.subjectRANK-
Palavras-chave: dc.subjectRANK-L-
Título: dc.titleClinical Implications of Immune Checkpoints and the RANK/RANK-L Signaling Pathway in High-Grade Canine Mast Cell Tumors-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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