Flavonoids modulate regenerative-related cellular events in LPS-challenged dental pulp cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMendes Soares, Igor Paulino-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorRibeiro, Rafael Antonio de Oliveira-
Autor(es): dc.creatorMota, Rafaella Lara Maia-
Autor(es): dc.creatorPires, Maria Luiza Barucci Araujo-
Autor(es): dc.creatorFernandes, Lídia de Oliveira-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorHebling, Josimeri-
Data de aceite: dc.date.accessioned2025-08-21T21:51:45Z-
Data de disponibilização: dc.date.available2025-08-21T21:51:45Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2024.105424-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305603-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305603-
Descrição: dc.descriptionObjective: To investigate the effects of quercetin (QU), hesperetin (HT), and taxifolin (TX) on human dental pulp cells (hDPCs) chronically exposed to lipopolysaccharide (LPS). Methods: First, the cytotoxicity (alamarBlue) and bioactivity (biomineralization, Alizarin Red) of QU, HT, and TX concentrations were evaluated on healthy hDPCs. Then, the effects of non-cytotoxic and bioactive concentrations were investigated on hDPCs after previous stimulation with E. coli LPS (10 µg/mL) for 7 days. Cell culture media with and without LPS were used as positive and negative controls, respectively. Cell viability (alamarBlue), NF-κB activation (immunofluorescence), reactive oxygen species production (ROS, H2DCFDA probe), cell migration (Transwell), inflammation-related gene expression (RT-qPCR), and odontogenic differentiation (RT-qPCR and alizarin red) were evaluated (n = 8). Data were analyzed using confidence intervals and ANOVA (α = 5 %). Results: The concentrations of 20 µM QU, 20 µM HT, and 200 µM TX reduced cell viability by more than 30 %. The 5 µM QU, 10 µM HT, and 100 µM TX concentrations were cytocompatible and stimulated biomineralization by healthy hDPCs. These concentrations were tested under the LPS challenge, and cell viability and odontogenic differentiation were significantly increased, while ROS production and inflammatory response were significantly decreased. In addition, the flavonoids significantly stimulated cell migration, reduced NF-κB activation, and increased biomineralization by LPS-challenged hDPCs compared to cells exposed to LPS alone and without any other treatment. Conclusion: Flavonoids can modulate the metabolism of hDPCs chronically exposed to LPS in vitro, stimulating cellular events compatible with stem cell-based regenerative processes. Clinical significance: Flavonoids may be explored as adjuvant therapeutic agents during pulp capping to counteract chronic inflammatory conditions and stimulate regeneration of the dentin-pulp complex in caries-affected teeth, thereby preserving tooth vitality.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry, São Paulo-
Descrição: dc.descriptionFAPESP: 2019/16473-1-
Descrição: dc.descriptionFAPESP: 2021/13096-2-
Descrição: dc.descriptionFAPESP: 2022/06603-8-
Descrição: dc.descriptionFAPESP: 2023/04465-0-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectGene expression, Mesenchymal stem cells-
Palavras-chave: dc.subjectLipopolysaccharides-
Palavras-chave: dc.subjectPolyphenols-
Palavras-chave: dc.subjectPulpitis-
Palavras-chave: dc.subjectRegenerative endodontics-
Título: dc.titleFlavonoids modulate regenerative-related cellular events in LPS-challenged dental pulp cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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