Functionalization of PCL-Based Fiber Scaffolds with Different Sources of Calcium and Phosphate and Odontogenic Potential on Human Dental Pulp Cells

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Michigan-
Autor(es): dc.contributorThe University of British Columbia (UBC)-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorMendes Soares, Igor Paulino-
Autor(es): dc.creatorMota, Rafaella Lara Maia-
Autor(es): dc.creatorLeite, Maria Luísa-
Autor(es): dc.creatorRibeiro, Rafael Antonio de Oliveira-
Autor(es): dc.creatorFernandes, Lídia de Oliveira-
Autor(es): dc.creatorBottino, Marco C.-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorHebling, Josimeri-
Data de aceite: dc.date.accessioned2025-08-21T16:01:16Z-
Data de disponibilização: dc.date.available2025-08-21T16:01:16Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/jfb15040097-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305597-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305597-
Descrição: dc.descriptionThis study investigated the incorporation of sources of calcium, phosphate, or both into electrospun scaffolds and evaluated their bioactivity on human dental pulp cells (HDPCs). Additionally, scaffolds incorporated with calcium hydroxide (CH) were characterized for degradation, calcium release, and odontogenic differentiation by HDPCs. Polycaprolactone (PCL) was electrospun with or without 0.5% w/v of calcium hydroxide (PCL + CH), nano-hydroxyapatite (PCL + nHA), or β-glycerophosphate (PCL + βGP). SEM/EDS analysis confirmed fibrillar morphology and particle incorporation. HDPCs were cultured on the scaffolds to assess cell viability, adhesion, spreading, and mineralized matrix formation. PCL + CH was also evaluated for gene expression of odontogenic markers (RT-qPCR). Data were submitted to ANOVA and Student’s t-test (α = 5%). Added CH increased fiber diameter and interfibrillar spacing, whereas βGP decreased both. PCL + CH and PCL + nHA improved HDPC viability, adhesion, and proliferation. Mineralization was increased eightfold with PCL + CH. Scaffolds containing CH gradually degraded over six months, with calcium release within the first 140 days. CH incorporation upregulated DSPP and DMP1 expression after 7 and 14 days. In conclusion, CH- and nHA-laden PCL fiber scaffolds were cytocompatible and promoted HDPC adhesion, proliferation, and mineralized matrix deposition. PCL + CH scaffolds exhibit a slow degradation profile, providing sustained calcium release and stimulating HDPCs to upregulate odontogenesis marker genes.-
Descrição: dc.descriptionDepartment of Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Cariology Restorative Sciences and Endodontics School of Dentistry University of Michigan-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Oral Health Sciences Faculty of Dentistry The University of British Columbia (UBC)-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Morphology Orthodontics and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Functional Biomaterials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcalcium-
Palavras-chave: dc.subjectcell-homing therapy-
Palavras-chave: dc.subjectphosphates-
Palavras-chave: dc.subjectpulp capping agents-
Palavras-chave: dc.subjectscaffolds-
Palavras-chave: dc.subjecttissue engineering-
Título: dc.titleFunctionalization of PCL-Based Fiber Scaffolds with Different Sources of Calcium and Phosphate and Odontogenic Potential on Human Dental Pulp Cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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