Antimicrobial efficacy of alternative root canal disinfection strategies: An evaluation on multiple working models

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Autor(es): dc.contributorUniversity of Groningen-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPedrinha, Victor Feliz-
Autor(es): dc.creatorBarros, Mirela Cesar-
Autor(es): dc.creatorPortes, Juan Domingos-
Autor(es): dc.creatorSlomp, Anje Margje-
Autor(es): dc.creatorWoudstra, Willem-
Autor(es): dc.creatorLameira, Osmar Alves-
Autor(es): dc.creatorQueiroga, Carmen Lucia-
Autor(es): dc.creatorMarcucci, Maria Cristina-
Autor(es): dc.creatorShahbazi, Mohammad-Ali-
Autor(es): dc.creatorSharma, Prashant Kumar-
Autor(es): dc.creatorAndrade, Flaviana Bombarda de-
Data de aceite: dc.date.accessioned2025-08-21T18:32:27Z-
Data de disponibilização: dc.date.available2025-08-21T18:32:27Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-01-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2025.117833-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305538-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305538-
Descrição: dc.descriptionResearching disinfection strategies is pivotal because effectively eliminating bacteria and their byproducts during root canal treatment (RCT) remains a challenge. This study investigated the antimicrobial efficacy of natural antimicrobial compounds, propolis (PRO) and copaiba oil-resin (COR), compared to conventional agents in Endodontics. Antimicrobials were tested against endodontic pathogens via macrodilution with standardized inoculums to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Biofilm killing efficacy were performed using two dual-species biofilms: Enterococcus faecalis (ATCC 29212) and Streptococcus mutans (ATCC 20523) and Streptococcus oralis (J22) and Actinomyces naeslundii (T14V-J1) grown on dentine discs. At the intratubular level (dentine cylinders), dentine tubule contamination was performed with E. faecalis and S. mutans. The specimens were exposed to antimicrobials to simulate their use at different sets of RCT and bacterial viability was quantified using Live/Dead staining via confocal laser scanning microscopy (CLSM). Biofilm characteristics and immediate removal of S. oralis and A. naeslundii biofilm model were evaluated employing optical coherence tomography (OCT) and CFU/mL counting. Statistical tests were applied according to data distribution for each analysis (α=0.05). Macrodilutions showed different effects against endodontic pathogens. Direct contact and intratubular analysis showed that PRO and COR promoted disinfection like conventional agents (p > 0.05). According to OCT analysis, PRO and COR showed similar biofilm reduction after immediate contact (p < 0.05). CFU/mL counting showed decontamination (p < 0.05) after using natural and conventional agents. PRO and COR showed antimicrobial effects, indicating their suitability as complementary approaches in RCT to eliminate as much microbial load as possible.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Biomaterials and Biomedical Technology (BBT) University Medical Center Groningen (UMCG) University of Groningen-
Descrição: dc.descriptionDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University of São Paulo (FOB – USP), São Paulo-
Descrição: dc.descriptionLaboratory of Biotechnology EMBRAPA Eastern Amazon, Pará-
Descrição: dc.descriptionState University of Campinas CPQBA Chemistry of Natural Products Division, São Paulo-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionFAPESP: 2010/20186-3-
Descrição: dc.descriptionFAPESP: 2020/ 12726-0-
Idioma: dc.languageen-
Relação: dc.relationBiomedicine and Pharmacotherapy-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectDental pulp diseases-
Palavras-chave: dc.subjectEndodontics-
Palavras-chave: dc.subjectRoot canal irrigants-
Palavras-chave: dc.subjectRoot canal medications-
Palavras-chave: dc.subjectRoot canal preparation-
Título: dc.titleAntimicrobial efficacy of alternative root canal disinfection strategies: An evaluation on multiple working models-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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