Transdentinal effects of S-PRG fillers on odontoblast-like cells

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMendes Soares, Igor Paulino-
Autor(es): dc.creatorAnselmi, Caroline-
Autor(es): dc.creatorFernandes, Lídia de Oliveira-
Autor(es): dc.creatorPeruchi, Victoria-
Autor(es): dc.creatorde Lima, Caroline Meronha-
Autor(es): dc.creatorPires, Maria Luiza Barucci Araujo-
Autor(es): dc.creatorRibeiro, Rafael Antonio de Oliveira-
Autor(es): dc.creatorde Souza Costa, Carlos Alberto-
Autor(es): dc.creatorHebling, Josimeri-
Data de aceite: dc.date.accessioned2025-08-21T21:11:57Z-
Data de disponibilização: dc.date.available2025-08-21T21:11:57Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.dental.2024.06.002-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305536-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305536-
Descrição: dc.descriptionObjectives: To investigate the transdentinal effects of surface reaction-type pre-reacted glass-ionomer (S-PRG) fillers on odontoblast-like cells. Methods: An eluate of S-PRG fillers was obtained by dissolving the particles in distilled water (1:1 m/v). Dentin discs with similar permeability were mounted into artificial pulp chambers and MDPC-23 cells were seeded on their pulpal surface. The occlusal surface was treated with (n = 10): ultrapure water (negative control – NC), hydrogen peroxide (positive control – PC), S-PRG eluate exposure for 1 min (S-PRG 1 min), or S-PRG filler eluate exposure for 30 min (S-PRG 30 min). After 24 h, cell viability (alamarBlue) and morphology (SEM) were evaluated. The extract obtained from transdentinal diffusion was applied to MDPC-23 pre-cultured in plates for another 24 h to evaluate viability (alamarBlue, 1, 3, and 7 days), gene expression of Col1a1, Alpl, Dspp, and Dmp1 (RT-qPCR, 1 and 7 days), and mineralization (Alizarin Red, 7 days). Data were analyzed with ANOVA (α = 5 %). Results: While S-PRG 1 min did not differ from NC, S-PRG 30 min reduced 17.9 % viability of cells from discs. S-PRG treatments resulted in low cell detaching from dentin, and the remaining cells exhibited typical morphology or minor cytoplasmic contraction. S-PRG 30 min slightly increased cell viability (6 %) 1 day after contact with the extract. S-PRG treatments upregulated the expression of the investigated genes, especially after 1 day. S-PRG 30 min stimulated mineralization activity by 39.7 %. Conclusions: S-PRG filler eluate does not cause transdentinal cytotoxicity on odontoblast-like cells, and long-term exposure can stimulate their dentinogenic-related mineralization activity. Significance: The transdentinal elution of ions from S-PRG fillers is not expected to be harmful to the dental pulp and may exert bioactive effects by inducing dentin matrix deposition through the metabolism of underlying odontoblasts.-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Morphology Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Restorative Dentistry São Paulo State University (UNESP) School of Dentistry-
Descrição: dc.descriptionDepartment of Physiology and Pathology São Paulo State University (UNESP) School of Dentistry-
Formato: dc.format1259-1266-
Idioma: dc.languageen-
Relação: dc.relationDental Materials-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiomineralization-
Palavras-chave: dc.subjectCell survival-
Palavras-chave: dc.subjectDentin-
Palavras-chave: dc.subjectIons-
Palavras-chave: dc.subjectOdontoblasts-
Palavras-chave: dc.subjectToxicity-
Título: dc.titleTransdentinal effects of S-PRG fillers on odontoblast-like cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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