PAPP-A enhances the antioxidative effects of IGF-1 during bovine in vitro embryo production

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Western São Paulo (Unoeste)-
Autor(es): dc.creatorFagali Franchi, Fernanda-
Autor(es): dc.creatorDos Santos, Priscila Helena-
Autor(es): dc.creatorKubo Fontes, Patricia-
Autor(es): dc.creatorValencise Quaglio, Ana Elisa-
Autor(es): dc.creatorGomes Nunes, Sarah-
Autor(es): dc.creatorZoccal Mingoti, Gisele-
Autor(es): dc.creatorde Souza Castilho, Anthony César-
Data de aceite: dc.date.accessioned2025-08-21T23:01:00Z-
Data de disponibilização: dc.date.available2025-08-21T23:01:00Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2024.07.016-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305461-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305461-
Descrição: dc.descriptionWe investigated whether exogenous pregnancy-associated plasma protein-A (PAPP-A) enhances the antioxidant role of insulin-like growth factor-1 (IGF-1) in bovine in vitro embryo production (IVP). We performed standard in vitro maturation (IVM) and in vitro culture (IVC) or added menadione to promote an oxidative stressed microenvironment and evaluated the antioxidant effect of IGF-1 alone or in combination with PAPP-A (IGF-1/PAPP-A). In IVM, the treatments did not affect oocyte nuclear development, total GSH content, cumulus cell gene expression, and blastocyst yield. Nevertheless, IGF-1/PAPP-A treatment prevented an increase in reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) levels. In IVC, the treatments did not affect the total GSH content on blastocysts and IVC media, but IGF-1 and IGF-1/PAPP-A treatments increased blastocyst yield compared to the menadione group. In addition, IGF-1/PAPP-A treatment had lower ROS levels and regulated genes related to embryonic quality compared to the control and menadione groups. Overall, we showed that PAPP-A could enhance the antioxidant role of IGF-1 during IVP in cattle by avoiding higher ROS levels in oocytes and blastocysts and modulating the transcriptional abundance of genes involved in oxidative protection and embryonic quality.-
Descrição: dc.descriptionSão Paulo State University (Unesp), Department of Pharmacology, Institute of Biosciences, Botucatu, São Paulo, Brazil; Reproductive and Developmental Biology Laboratory, Department of Veterinary Medicine and Animal Science, University of Milan, Milan, Italy-
Descrição: dc.descriptionSão Paulo State University (Unesp) Department of Pharmacology Institute of Biosciences-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Veterinary Medicine Laboratory of Reproductive Physiology-
Descrição: dc.descriptionUniversity of Western São Paulo (Unoeste) Department of Animal Science-
Descrição: dc.descriptionSão Paulo State University (Unesp) Department of Pharmacology Institute of Biosciences-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Veterinary Medicine Laboratory of Reproductive Physiology-
Formato: dc.format191-201-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCattle-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectInsulin-like growth factor-1-
Palavras-chave: dc.subjectOxidative stress-
Palavras-chave: dc.subjectPappalysin-
Palavras-chave: dc.subjectROS-
Título: dc.titlePAPP-A enhances the antioxidative effects of IGF-1 during bovine in vitro embryo production-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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