JARID1B represses the osteogenic potential of human periodontal ligament mesenchymal cells

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Autor(es): dc.contributorPaulista University-
Autor(es): dc.contributorPaulista University – UNIP-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Espírito Santo-
Autor(es): dc.creatorFerreira, Rogério S.-
Autor(es): dc.creatorda Silva, Rodrigo A.-
Autor(es): dc.creatorFeltran, Geórgia Da S.-
Autor(es): dc.creatorda Silva, Ericka Patricia-
Autor(es): dc.creatorde Assis, Rahyza I. F.-
Autor(es): dc.creatorRovai, Emanuel Silva-
Autor(es): dc.creatorZambuzzi, Willian F.-
Autor(es): dc.creatorAndia, Denise C.-
Data de aceite: dc.date.accessioned2025-08-21T16:34:16Z-
Data de disponibilização: dc.date.available2025-08-21T16:34:16Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/odi.14814-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305350-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305350-
Descrição: dc.descriptionBackground: Here, we evaluated whether the histone lysine demethylase 5B (JARID1B), is involved in osteogenic phenotype commitment of periodontal ligament cells (PDLCs), by considering their heterogeneity for osteoblast differentiation. Materials and Methods: Epigenetic, transcriptional, and protein levels of a gene set, involved in the osteogenesis, were investigated by performing genome-wide DNA (hydroxy)methylation, mRNA expression, and western blotting analysis at basal (without osteogenic induction), and at the 3rd and 10th days of osteogenic stimulus, in vitro, using PDLCs with low (l) and high (h) osteogenic potential as biological models. Results: h-PDLCs showed reduced levels of JARID1B, compared to l-PDLCs, with significant inversely proportional correlations between RUNX2 and RUNX2/p57. Epigenetically, a significant reduction in the global H3K4me3 content was observed only in h-PDLCs. Immunoblotting data reveal a significant reduction in the global H3K4me3 content, at 3 days of induction only in h-PDLCs, while an increase in the global H3K4me3 content was observed at 10 days for both PDLCs. Additionally, positive correlations were found between global H3K4me3 levels and JARID1B gene expression. Conclusions: Altogether, our results show the crucial role of JARID1B in repressing PDLCs osteogenic phenotype and this claims to pre-clinical protocols proposing JARID1B as a potential therapeutic target.-
Descrição: dc.descriptionSchool of Dentistry Health Science Institute Paulista University-
Descrição: dc.descriptionCEEpiRG Program in Environmental and Experimental Pathology Paulista University – UNIP-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry Lab. of Bioassays and Cellular Dynamics Institute of Biosciences UNESP – São Paulo State University-
Descrição: dc.descriptionDepartment of Clinical Dentistry Federal University of Espírito Santo-
Descrição: dc.descriptionDivision of Periodontics Institute of Science and Technology UNESP – São Paulo State University-
Descrição: dc.descriptionDepartment of Chemistry and Biochemistry Lab. of Bioassays and Cellular Dynamics Institute of Biosciences UNESP – São Paulo State University-
Descrição: dc.descriptionDivision of Periodontics Institute of Science and Technology UNESP – São Paulo State University-
Formato: dc.format3971-3981-
Idioma: dc.languageen-
Relação: dc.relationOral Diseases-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbone-
Palavras-chave: dc.subjectepigenetics-
Palavras-chave: dc.subjectJARID1B-
Palavras-chave: dc.subjectmesenchymal stem cells-
Palavras-chave: dc.subjectosteoblast-
Palavras-chave: dc.subjectPDLC-
Título: dc.titleJARID1B represses the osteogenic potential of human periodontal ligament mesenchymal cells-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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