Interleukin-27 Regulates Adaptative Immune Responses Associated With Control of Parasite Replication in Canine Leishmaniasis

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatordos Santos, Marilene Oliveira-
Autor(es): dc.creatorCosta, Sidnei Ferro-
Autor(es): dc.creatorRebech, Gabriela Torres-
Autor(es): dc.creatorde Freitas, Jéssica Henrique-
Autor(es): dc.creatorBragato, Jaqueline Poleto-
Autor(es): dc.creatorSoares, Matheus Fujimura-
Autor(es): dc.creatorIto, Lucas Takeshi Siqueira-
Autor(es): dc.creatorde Rezende Eugênio, Flavia-
Autor(es): dc.creatordos Santos, Paulo Sérgio Patto-
Autor(es): dc.creatorde Lima, Valéria Marçal Felix-
Data de aceite: dc.date.accessioned2025-08-21T15:43:04Z-
Data de disponibilização: dc.date.available2025-08-21T15:43:04Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/pim.13063-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305343-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305343-
Descrição: dc.descriptionInterleukin 27 (IL-27) is a cytokine that regulates susceptibility to Leishmania infantum infection in humans and experimental models. This cytokine has not yet been described in canine leishmaniasis (CanL). Therefore, we investigated whether IL-27 has a regulatory role in CanL. The EBI3 and p28 subunits of IL-27 were measured in splenic leukocytes culture supernatant from dogs with CanL and compared to control dogs. We also correlated EBI3 and p28 levels with IL-21, anti-L. infantum antibodies and parasite loads. We performed functional assays followed by IL-27 blockade and measured parasite loads, production of cytokines in splenic leukocytes culture supernatant, and the expression of PD-1, CTLA-4, phospho-Stat-1/3, T-bet, GATA3 and nitric oxide production (NO). Both IL-27 subunits increased in the supernatant of dogs with CanL compared to control dogs. EBI3 and p28 levels showed a moderate positive correlation with IL-21 (r = 0.67, p < 0.0001 and r = 0.45, p < 0.012, respectively), and the EBI3 subunit was positively associated with anti-L. infantum IgG antibodies (r = 0.38, p < 0.040) and parasite load (r = 0.47, p < 0.009). IL-27 and IL-21 participate of immune responses in CanL. IL-27 may be associated with the failure of immunity to control parasite replication via upregulation of the expression of PD-1, CTLA-4, T-bet and NO in splenic leukocytes from dogs with CanL. These findings suggest that the pathways regulated by IL-27 are involved in CanL pathogenesis in the host, and may be targets for new therapies.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Clinics Surgery and Animal Reproduction São Paulo State University (UNESP)-
Descrição: dc.descriptionDepartment of Clinics Surgery and Animal Reproduction São Paulo State University (UNESP)-
Descrição: dc.descriptionFAPESP: 2019/18252-2-
Idioma: dc.languageen-
Relação: dc.relationParasite Immunology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectdogs-
Palavras-chave: dc.subjectIL-27-
Palavras-chave: dc.subjectimmunity-
Palavras-chave: dc.subjectspleen-
Palavras-chave: dc.subjectvisceral leishmaniasis-
Título: dc.titleInterleukin-27 Regulates Adaptative Immune Responses Associated With Control of Parasite Replication in Canine Leishmaniasis-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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