Cryopreservation and transplantation of spermatogonia stem cells in piracanjuba Brycon orbignyanus (Characiformes: Characidae), an endangered fish species

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorChico Mendes Institute of Biodiversity Conservation/National Center for Research and Conservation of Continental Aquatic Biodiversity-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorFederal University of the South and Southeast of Pará-
Autor(es): dc.contributorChina Three Gorges Corporation (CTG)-
Autor(es): dc.contributorCentro de Investigación Científica y de Educación Superior de Ensenada (CICESE)-
Autor(es): dc.creatorLópez, Lucia Suárez-
Autor(es): dc.creatorMonzani, Paulo Sérgio-
Autor(es): dc.creatorCarvalho, Gabriella Braga-
Autor(es): dc.creatorde Siqueira Silva, Diógenes Henrique-
Autor(es): dc.creatorVianna, Norberto Castro-
Autor(es): dc.creatorYasui, George Shigueki-
Autor(es): dc.creatorSenhorini, José Augusto-
Data de aceite: dc.date.accessioned2025-08-21T17:54:31Z-
Data de disponibilização: dc.date.available2025-08-21T17:54:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s10695-024-01406-6-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305304-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305304-
Descrição: dc.descriptionPiracanjuba (Brycon orbignyanus) is an endangered fish species from the Neotropical region. The establishment of a cryobank using spermatogonial stem cells (SSCs) and subsequent production of a germline chimera is thus a promising strategy for such species. In the present work, procedures for the isolation and cryopreservation of piracanjuba SSCs and subsequent transplantation into sterile recipients were established. The piracanjuba SSCs were obtained by Percoll density gradient centrifugation and differential plating. SSC fractions were evaluated by relative ddx4 expression, alkaline phosphatase activity, and light microscopy. SSC cryopreservation was performed using five cryoprotectants at three different concentrations. The mix of the cells from the 20% and 30% Percoll density gradients showed 58.35 ± 0.03% purity of SSCs. The purity of SSCs increased to 66.00 ± 0.01% after differential plating. The relative ddx4 expression was 3.5 times higher in cells from the Percoll density gradient centrifugation than in the gonad and cells after differential plating. Propanediol (1 M) was the most effective cryoprotector evaluated (P = 1.000), showing 90.75 ± 1.85% cell viability. Freshly isolated and cryopreserved cells from the Percoll density gradient centrifugation were transplanted into a sterile male adult triploid hybrid with germ cell-less gonads. SSCs were observed in the germinal epithelium of the testes of recipients 20 days after transplantation. The results are promising for obtaining functional germline chimeras in Neotropical fish. Consequently, although the number of males used for the experiment was borderline, the procedures established here can be applied in future actions for the conservation and reconstitution of the piracanjuba in case of extinction.-
Descrição: dc.descriptionInstitute of Bioscience São Paulo State University, São Paulo-
Descrição: dc.descriptionLaboratory of Fish Biotechnology Chico Mendes Institute of Biodiversity Conservation/National Center for Research and Conservation of Continental Aquatic Biodiversity, São Paulo-
Descrição: dc.descriptionDepartment of Animal Reproduction School of Veterinary Medicine and Animal Science University of São Paulo, São Paulo-
Descrição: dc.descriptionStudy Group on the Reproduction of Amazonian Fishes Biology Faculty Federal University of the South and Southeast of Pará, Pará-
Descrição: dc.descriptionChina Three Gorges Corporation (CTG), São Paulo-
Descrição: dc.descriptionDepartment of Aquaculture Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), No. 3918. Zona Playitas, Carretera Ensenada, Baja California-
Descrição: dc.descriptionInstitute of Bioscience São Paulo State University, São Paulo-
Formato: dc.format2117-2135-
Idioma: dc.languageen-
Relação: dc.relationFish Physiology and Biochemistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectConservation-
Palavras-chave: dc.subjectGene bank-
Palavras-chave: dc.subjectGermline chimera-
Palavras-chave: dc.subjectSpermatogonia-
Palavras-chave: dc.subjectTransplantation-
Título: dc.titleCryopreservation and transplantation of spermatogonia stem cells in piracanjuba Brycon orbignyanus (Characiformes: Characidae), an endangered fish species-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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