Cell sheet produced from periodontal ligament stem cells activated by PAR1 improves osteogenic differentiation

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorSchool of Dentistry-
Autor(es): dc.contributorDivision of Comprehensive Oral Health-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorWilliam Harvey Research Institute-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorGasparoni, Letícia Miquelitto-
Autor(es): dc.creatorAlves, Tomaz-
Autor(es): dc.creatorFrança, Bruno Nunes de-
Autor(es): dc.creatorBalzarini, Danilo-
Autor(es): dc.creatorAlbuquerque-Souza, Emmanuel-
Autor(es): dc.creatorPedroni, Ana Clara Fagundes-
Autor(es): dc.creatorRovai, Emanuel da Silva-
Autor(es): dc.creatorMendoza, Aldrin Huamán-
Autor(es): dc.creatorSipert, Carla Renata-
Autor(es): dc.creatorHolzhausen, Marinella-
Data de aceite: dc.date.accessioned2025-08-21T18:56:06Z-
Data de disponibilização: dc.date.available2025-08-21T18:56:06Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1590/1807-3107bor-2024.vol38.0079-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305248-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305248-
Descrição: dc.descriptionPeriodontal regeneration is a challenge, and tissue engineering based on periodontal ligament stem cells (PDLSCs) has been shown to be a promising alternative to this process. However, the need for scaffolds has limited the therapeutic use of PDLSCs. In this context, scaffold-free tissue engineering using the cell sheet (CS) technique has been developed as an alternative approach to improve tissue regeneration. Previously, we showed that Protease-activated receptor-1 (PAR1) can regulate PDLSCs. Herein, we evaluate whether PAR1 influences osteogenesis in CSs produced from PDLSCs, without the use of scaffolds. PDLSCs were isolated and immunophenotyped. Then, CSs were obtained by supplementing the culture medium with ascorbic acid (50 µg/mL), and PAR1 was activated through its agonist peptide (100 nM). Scaffold-free 3D CSs were successfully produced from PDLSCs, and they showed higher proliferation potential than isolated PDLSCs. Also, PAR1 activation decreased senescence and improved osteogenic differentiation of CSs by increasing mineralized nodule deposition and alkaline phosphatase concentration; PAR1 also modulated osteogenic markers at the gene and protein levels. We further demonstrated that this effect was regulated by Wnt, TGF-βI, MEK, p38 MAPK, and FGF/VEGF signaling pathways in PDLSCs (p < 0.05%). Overall, PAR1 activation increased osteogenic activity in CSs, emerging as a promising scaffold-free therapeutic approach for periodontal regeneration.-
Descrição: dc.descriptionUniversidade Federal de Juiz de Fora UFJF School of Dentistry Department of Dental Clinic, MG-
Descrição: dc.descriptionUniversity of North Carolina Adams School of Dentistry Division of Comprehensive Oral Health-
Descrição: dc.descriptionUniversidade São Francisco – USF School of Dentistry, Bragança Paulista, SP-
Descrição: dc.descriptionUniversidade de São Paulo – USP School of Dentistry Department of Stomatology, SP-
Descrição: dc.descriptionQueen Mary University William Harvey Research Institute-
Descrição: dc.descriptionUniversidade de São Paulo – USP School of Dentistry Department of Restorative Dentistry, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista – Unesp Institute of Science and Technology Division of Periodontics, SP-
Descrição: dc.descriptionUniversidade Estadual Paulista – Unesp Institute of Science and Technology Division of Periodontics, SP-
Idioma: dc.languageen-
Relação: dc.relationBrazilian Oral Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell Culture Techniques-
Palavras-chave: dc.subjectOsteogenesis-
Palavras-chave: dc.subjectPeriodontal Ligament-
Palavras-chave: dc.subjectProtease-Activated-
Palavras-chave: dc.subjectReceptors-
Palavras-chave: dc.subjectStem Cells-
Título: dc.titleCell sheet produced from periodontal ligament stem cells activated by PAR1 improves osteogenic differentiation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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