Mechanistic insights on the antibacterial action of the kyotorphin peptide derivatives revealed by in vitro studies and Galleria mellonella proteomic analysis

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de São Paulo (UNIFESP)-
Autor(es): dc.contributorUniversitat de Girona-
Autor(es): dc.contributorFaculdade de Medicina de Lisboa-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorde Andrade, Vitor M.-
Autor(es): dc.creatorde Oliveira, Vitor D.M.-
Autor(es): dc.creatorBarcick, Uilla-
Autor(es): dc.creatorRamu, Vasanthakumar G.-
Autor(es): dc.creatorHeras, Montserrat-
Autor(es): dc.creatorBardají, Eduard R.-
Autor(es): dc.creatorCastanho, Miguel A.R.B.-
Autor(es): dc.creatorZelanis, André-
Autor(es): dc.creatorCapella, Aline-
Autor(es): dc.creatorJunqueira, Juliana C.-
Autor(es): dc.creatorConceição, Katia-
Data de aceite: dc.date.accessioned2025-08-21T16:08:37Z-
Data de disponibilização: dc.date.available2025-08-21T16:08:37Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.micpath.2024.106607-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/305150-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/305150-
Descrição: dc.descriptionObjectives: The selected kyotorphin derivatives were tested to improve their antimicrobial and antibiofilm activity. The antimicrobial screening of the KTP derivatives were ascertained in the representative strains of bacteria, including Streptococcus pneumoniae, Streptococcus pyogenes, Escherichia coli and Pseudomonas aeruginosa. Methods: Kyotorphin derivatives, KTP-NH2, KTP–NH2–DL, IbKTP, IbKTP-NH2, MetKTP-DL, MetKTP-LD, were designed and synthesized to improve lipophilicity and resistance to enzymatic degradation. Peptides were synthesized by standard solution or solid-phase peptide synthesis and purified using RP-HPLC, which resulted in >95 % purity, and were fully characterized by mass spectrometry and 1H NMR. The minimum inhibitory concentrations (MIC) determined for bacterial strains were between 20 and 419 μM. The direct effect of IbKTP-NH2 on bacterial cells was imaged using scanning electron microscopy. The absence of toxicity, high survival after infection and an increase in the hemocytes count was evaluated by injections of derivatives in Galleria mellonella larvae. Proteomics analyses of G. mellonella hemolymph were performed to investigate the underlying mechanism of antibacterial activity of IbKTP-NH2 at MIC. Results: IbKTP-NH2 induces morphological changes in bacterial cell, many differentially expressed proteins involved in DNA replication, synthesis of cell wall, and virulence were up-regulated after the treatment of G. mellonella with IbKTP-NH2. Conclusion: We suggest that this derivative, in addition to its physical activity on the bacterial membranes, can elicit a cellular and humoral immune response, therefore, it could be considered for biomedical applications.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratório de Bioquímica de Peptídeos Departamento de Ciência e Tecnologia Universidade Federal de São Paulo – UNIFESP, Rua Talim, 330-
Descrição: dc.descriptionLaboratório de Proteômica Funcional Departamento de Ciência e Tecnologia Universidade Federal de São Paulo – Universidade Federal de São Paulo – UNIFESP, Rua Talim, 330-
Descrição: dc.descriptionLaboratori d'Innovació en Processos i Productes de Síntesi Orgànica (LIPPSO) Departament de Química Universitat de Girona, Campus Montilivi-
Descrição: dc.descriptionInstituto de Medicina Molecular Faculdade de Medicina de Lisboa, Av. Professor Egas Moniz-
Descrição: dc.descriptionLaboratório ProLaser Departamento de Ciência e Tecnologia Universidade Federal de São Paulo – UNIFESP, Rua Talim, 330-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), SP-
Descrição: dc.descriptionDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (Unesp), SP-
Descrição: dc.descriptionFAPESP: 2021/04316–9-
Idioma: dc.languageen-
Relação: dc.relationMicrobial Pathogenesis-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntimicrobial-
Palavras-chave: dc.subjectBiofilm-
Palavras-chave: dc.subjectGalleria mellonella-
Palavras-chave: dc.subjectIbuprofen-
Palavras-chave: dc.subjectKyotorphin-
Palavras-chave: dc.subjectProteome-
Título: dc.titleMechanistic insights on the antibacterial action of the kyotorphin peptide derivatives revealed by in vitro studies and Galleria mellonella proteomic analysis-
Tipo de arquivo: dc.typelivro digital-
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