Dose-dependent effects of zoledronic acid on the osteogenic differentiation of human bone marrow stem cells (hBMSCs)

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorHarvard School of Dental Medicine-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorHarvard Medical School-
Autor(es): dc.creatorHadad, Henrique-
Autor(es): dc.creatorMatheus, Henrique Rinaldi-
Autor(es): dc.creatorChen, Jason Evan-
Autor(es): dc.creatorJounaidi, Youssef-
Autor(es): dc.creatorSouza, Francisley Ávila-
Autor(es): dc.creatorGuastaldi, Fernando Pozzi Semeghini-
Data de aceite: dc.date.accessioned2025-08-21T20:47:33Z-
Data de disponibilização: dc.date.available2025-08-21T20:47:33Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jormas.2023.101479-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304803-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304803-
Descrição: dc.descriptionRecent studies have shown that bisphosphonates can also impact osteoblasts besides osteoclasts. This study aimed to evaluate the effects of different concentrations of Zoledronic acid (ZA) during the osteogenic differentiation of human Bone Marrow Stem Cells (hBMSCs) in vitro. Thus, osteogenic differentiation of hBMSCs was conducted with different concentrations of Zoledronic Acid (ZA) (0, 0.1, 1.0, and 5.0 μM) for the first 3 days. Cell metabolism was quantified at 1-, 3-, 7-, and 14 days. At 7- and 14-days, the following analyses were performed: 1) mineralization nodule assay, 2) LIVE/DEAD™, 3) cell adhesion and spreading, 4) alkaline phosphatase (ALP) activity, and 5) qPCR analysis for RUNX-2), ALPL, and COL1 A1. Data were analyzed by ANOVA 2-way, followed by Tukey's post hoc test (p < 0.05). Cell metabolism (3-, 7-, and 14-days) (p < 0.001), mineralization (7-, 14-days) (p < 0.001), and ALP activity (14-days) (p < 0.001) were reduced in ZA 5.0 µM when compared to control (no ZA). Also, ZA 5.0 µM downregulated the expression of RUNX2 at 7- and 14-days (p < 0.001). It is possible to conclude that ZA (5.0 µM) can impair hBMSC differentiation into osteoblasts and interferes with its mineralization phase.-
Descrição: dc.descriptionSkeletal Biology Research Center Department of Oral and Maxillofacial Surgery Massachusetts General Hospital Harvard School of Dental Medicine-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Oral & Maxillofacial Surgery Division São Paulo State University (UNESP) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Division of Periodontics São Paulo State University (UNESP) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Harvard Medical School-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Oral & Maxillofacial Surgery Division São Paulo State University (UNESP) School of Dentistry, SP-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery Division of Periodontics São Paulo State University (UNESP) School of Dentistry, SP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Stomatology, Oral and Maxillofacial Surgery-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectHuman bone marrow stem cells-
Palavras-chave: dc.subjectMedication-related osteonecrosis of the jaw-
Palavras-chave: dc.subjectOsteogenic differentiation-
Palavras-chave: dc.subjectZoledronic acid-
Título: dc.titleDose-dependent effects of zoledronic acid on the osteogenic differentiation of human bone marrow stem cells (hBMSCs)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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