Pre-fertilization approach using α-l-fucosidase modulates zona pellucida hardening during bovine in vitro embryo production

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversity of Western São Paulo (UNOESTE)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorManssur, Thainá Sallum Bacco-
Autor(es): dc.creatorSebastião, Talita Raquel Cavichioli-
Autor(es): dc.creatorFranchi, Fernanda Fagali-
Autor(es): dc.creatordos Santos, Priscila Helena-
Autor(es): dc.creatorRazza, Eduardo Montanari-
Autor(es): dc.creatorNunes, Sarah Gomes-
Autor(es): dc.creatorCastilho, Anthony César de Souza-
Autor(es): dc.creatorFontes, Patricia Kubo-
Data de aceite: dc.date.accessioned2025-08-21T21:33:10Z-
Data de disponibilização: dc.date.available2025-08-21T21:33:10Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-04-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1007/s11259-023-10291-y-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304645-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304645-
Descrição: dc.descriptionThe polyspermy occurrence is considerably lower under in vivo compared to in vitro embryo culture conditions, suggesting that the presence of some factors in the maternal environment is responsible for this. The α-L-fucosidase (FUCA) is a natural glycosidase present in the oviductal fluid, therefore, this study aimed at investigating the effect of adding FUCA to the hardening of the zona pellucida (ZP), polyspermy control, and embryonic yield and quality of bovine blastocysts produced in vitro. In the first experiment, the effect of FUCA (0.125 U/mL) was evaluated during the entire in vitro fertilization (IVF). However, it was demonstrated to be embryotoxic by completely inhibiting the blastocyst formation. In the second experiment, the FUCA (0.125 U/mL) was tested as short-term incubation before IVF (pre-fertilization step) for 30 min or 2 h, which demonstrated that FUCA treatment for 30 min resulted in ZP hardening. In the third experiment, a pre-fertilization FUCA treatment (1 h) at different concentrations (0, 0.0625, and 0.125 U/mL) showed that FUCA (0.0625 U/mL) improved pre-fertilization ZP hardening and tended to increase monospermic fertilization rates but did not improve embryo yield and quality. Together, it has been demonstrated that FUCA can induce oocyte pre-fertilization ZP hardening and might improve monospermic fertilization performance, and this effect is dependent on both variables (protein concentration and incubation time).-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionUniversity of Western São Paulo (UNOESTE), São Paulo State-
Descrição: dc.descriptionLaboratory of Phytomedicines Pharmacology and Biotechnology Department of Pharmacology Institute of Biosciences University of São Paulo State (UNESP), Rua Prof. Antonio Celso Wagner Zanin, S/N, Zip Code: 18618689, São Paulo State-
Descrição: dc.descriptionLaboratory of Phytomedicines Pharmacology and Biotechnology Department of Pharmacology Institute of Biosciences University of São Paulo State (UNESP), Rua Prof. Antonio Celso Wagner Zanin, S/N, Zip Code: 18618689, São Paulo State-
Descrição: dc.descriptionFAPESP: 2016/25685-4-
Descrição: dc.descriptionFAPESP: 2018/06674-7-
Descrição: dc.descriptionFAPESP: 2019/12223-0-
Descrição: dc.descriptionCAPES: Finance Code 001-
Formato: dc.format1135-1147-
Idioma: dc.languageen-
Relação: dc.relationVeterinary Research Communications-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBlastocyst-
Palavras-chave: dc.subjectCattle-
Palavras-chave: dc.subjectFUCA-
Palavras-chave: dc.subjectGene Expression-
Palavras-chave: dc.subjectPolyspermy-
Título: dc.titlePre-fertilization approach using α-l-fucosidase modulates zona pellucida hardening during bovine in vitro embryo production-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.