Description and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorLizana, Rony Riveros-
Autor(es): dc.creatorCamargos, Rosiane de Souza-
Autor(es): dc.creatorSilva, Raully Lucas-
Autor(es): dc.creatorLeme, Bruno Balbino-
Autor(es): dc.creatorSakomura, Nilva Kazue-
Data de aceite: dc.date.accessioned2025-08-21T16:32:12Z-
Data de disponibilização: dc.date.available2025-08-21T16:32:12Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ani13162675-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304612-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304612-
Descrição: dc.descriptionIndirect calorimetry (IC) is a widely used method to study animal energy metabolism by measuring gas exchange. The accuracy of IC depends on detecting variations in signals reflecting the metabolic response, which can be challenging due to measurement noise and external factors. This study proposes a methodology to validate IC systems, including an easy-to-use spreadsheet for data computing, to verify accuracy and detect whole-system leaks. We conducted a recovery test using a simulation of CO2 dynamics in MS Excel and injecting a known CO2 concentration into four respirometry chambers. The thought flow rate of CO2 was observed and compared to the expected rate from the simulation. Data filtering and computing, including a detailed calculation of signals calibration, Bartholomew transformation, and noise reduction, was developed to obtain the gas exchange and heat production parameters using an open-circuit IC system. The results from the recovery test in our system show that the proposed methodology is accurate and precise. The proposed methodology and recovery test can be used to standardize the validation of IC systems together with adequate data computing, providing accurate measurements of animal energy metabolism in different environmental conditions and energy utilization from feeds.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionFaculty of Agricultural and Veterinary Sciences Sao Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, SP-
Descrição: dc.descriptionFaculty of Agricultural and Veterinary Sciences Sao Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, SP-
Descrição: dc.descriptionFAPESP: 2019/26575-6-
Idioma: dc.languageen-
Relação: dc.relationAnimals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectenergy expenditure-
Palavras-chave: dc.subjectfarm animals-
Palavras-chave: dc.subjectgas exchange-
Palavras-chave: dc.subjectmetabolic rate-
Palavras-chave: dc.subjectrespirometric chambers-
Título: dc.titleDescription and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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