Cytotoxic and Antibiofilm Properties of Antibiotic-Loaded Thermoresponsive Hydrogels for Root Canal Therapy

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Autor(es): dc.contributorCentre for Interdisciplinary Research in Health (CIIS)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorDuque, Cristiane-
Autor(es): dc.creatorBraga, Gabriela Pacheco de Almeida-
Autor(es): dc.creatorde Carvalho, Juliana Machado-
Autor(es): dc.creatorCaiaffa, Karina Sampaio-
Autor(es): dc.creatorNunes, Gabriel Pereira-
Autor(es): dc.creatorRabelo, Rafaela Laruzo-
Autor(es): dc.creatordos Santos, Vanessa Rodrigues-
Autor(es): dc.creatorPeres, Geórgia Rondó-
Autor(es): dc.creatorRibeiro, Lucas da Silva-
Autor(es): dc.creatorde Camargo, Emerson Rodrigues-
Data de aceite: dc.date.accessioned2025-08-21T17:32:34Z-
Data de disponibilização: dc.date.available2025-08-21T17:32:34Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2025-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/pr13030661-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304605-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304605-
Descrição: dc.descriptionDental infections can disrupt root development in immature permanent teeth, making traditional endodontic treatment challenging. Apexogenesis, a regenerative approach that promotes natural root development, offers a potential solution. However, issues related to disinfection and material biocompatibility still remain. The objective of this study was to evaluate the synergistic antimicrobial and antibiofilm properties of double and triple antibiotic combinations against common oral pathogens, and to incorporate the most effective combination into a thermosensitive hydrogel, to develop an alternative intracanal medication. Antibiotics were tested alone and in combination in planktonic and biofilm conditions of oral bacteria and Candida albicans. The antibiotic combinations with potential antimicrobial synergy were tested on Enterococcus faecalis biofilms in radicular dentin by confocal microscopy. Metronidazole (ME), ciprofloxacin (CI), and fosfomycin (FO) were incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogels, and their antibiofilm activity was compared to PNVCL hydrogels containing chlorhexidine (CHX) or calcium hydroxide (CH). The cytotoxicity of the hydrogels was assessed on MDPC-23 odontoblast-like cells using metiltetrazolium assays. A statistical analysis was performed using ANOVA followed by Tukey’s test (p < 0.05). The combination of ME + CI + FO showed superior antibiofilm effects in mono- and dual-species biofilms and on biofilms inside dentinal tubules, comparable to CHX. PNVCL hydrogels with ME + CI + FO significantly reduced E. faecalis biofilms in dentinal tubules, exhibiting a higher efficacy than PNVCL + CH. Cytotoxicity tests revealed minimal effects on cell viability for both PNVCL hydrogels with and without antibiotics. In conclusion, ME + CI + FO showed potent antimicrobial synergy and, when loaded in thermosensitive PNVCL hydrogel, demonstrated significant antibiofilm activity and low cytotoxicity. These findings emphasize the potential of this formulation as an effective and biocompatible endodontic medication, especially for the treatment of immature permanent teeth.-
Descrição: dc.descriptionUniversidade Católica Portuguesa (UCP) Faculty of Dental Medicine Centre for Interdisciplinary Research in Health (CIIS), Rua da Circunvalação, s/n-
Descrição: dc.descriptionSão Paulo State University (UNESP) Araçatuba Dental School Department of Preventive and Restorative Dentistry-
Descrição: dc.descriptionFederal University of São Carlos (UFSCar) Department of Chemistry, São Carlos-
Descrição: dc.descriptionSão Paulo State University (UNESP) Araçatuba Dental School Department of Preventive and Restorative Dentistry-
Idioma: dc.languageen-
Relação: dc.relationProcesses-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantibiotics-
Palavras-chave: dc.subjectcalcium hydroxide-
Palavras-chave: dc.subjectcell culture-
Palavras-chave: dc.subjectchlorhexidine-
Palavras-chave: dc.subjectcytotoxicity-
Palavras-chave: dc.subjecthydrogel-
Título: dc.titleCytotoxic and Antibiofilm Properties of Antibiotic-Loaded Thermoresponsive Hydrogels for Root Canal Therapy-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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