Development and evaluation of a colorimetric LAMP based-assay targeting the Bacteroides HF183 marker for tracking sewage pollution in environmental waters

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorEcosciences Precinct-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorXiamen University-
Autor(es): dc.contributorLouisiana State University-
Autor(es): dc.creatordo Nascimento, Mariah C.A.-
Autor(es): dc.creatorSmith, Wendy J.M.-
Autor(es): dc.creatorGebrewold, Metasebia-
Autor(es): dc.creatorLiu, Yawen-
Autor(es): dc.creatorSimpson, Stuart L.-
Autor(es): dc.creatorBivins, Aaron-
Autor(es): dc.creatorRahal, Paula-
Autor(es): dc.creatorAhmed, Warish-
Data de aceite: dc.date.accessioned2025-08-21T22:37:33Z-
Data de disponibilização: dc.date.available2025-08-21T22:37:33Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.watres.2024.122202-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304593-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304593-
Descrição: dc.descriptionSurface waters are vulnerable to contamination by human and animal feces, posing risks to human health due to potential exposure to enteric pathogens. This research developed a colorimetric loop-mediated isothermal amplification (cLAMP) assay to detect sewage associated Bacteroides dorei HF183/BacR287 (HF183) marker in wastewater and environmental water samples. The host sensitivity and host specificity of the assay were evaluated, and their performance was compared to the Bacteroides HF183 qPCR assay using control materials (gBlocks), environmental water samples seeded with untreated sewage, and ambient environmental water samples. In serial dilutions of control materials, qPCR produced quantifiable data across all dilutions, while cLAMP detected the marker down to 0.001 pg/µL of control materials, which was two orders of magnitude less sensitive than qPCR. All untreated sewage samples (n = 12) tested positive for HF183 by both the qPCR and cLAMP assays, demonstrating a host sensitivity value of 1.00 (maximum value of 1.00). The host specificity by analysing 70 non-human fecal nucleic acid samples revealed cLAMP's specificity value of 0.81 compared to qPCR's 0.64. When testing sewage-seeded environmental water samples, both methods detected HF183 for the lowest amount of sewage, indicating similar detection sensitivity. The application of cLAMP for tracking sewage pollution in environmental waters showed promising results, with moderate agreement between cLAMP and qPCR (κ = 0.510). However, cLAMP occasionally missed detections compared to qPCR, particularly in low-concentration samples. Overall, the cLAMP HF183 assay demonstrated promising potential as a rapid and sensitive method for detecting sewage pollution, offering a viable alternative to qPCR in certain environmental monitoring scenarios.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionCSIRO Environment Ecosciences Precinct, 41 Boggo Road-
Descrição: dc.descriptionDepartment of Biology São Paulo State University - UNESP São José do Rio Preto-
Descrição: dc.descriptionState Key Laboratory of Marine Environmental Science College of the Environment & Ecology Xiamen University-
Descrição: dc.descriptionDepartment of Civil & Environmental Engineering Louisiana State University-
Descrição: dc.descriptionDepartment of Biology São Paulo State University - UNESP São José do Rio Preto-
Idioma: dc.languageen-
Relação: dc.relationWater Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBacteroides HF183-
Palavras-chave: dc.subjectColorimetric LAMP-
Palavras-chave: dc.subjectEnvironmental water-
Palavras-chave: dc.subjectFecal pollution-
Palavras-chave: dc.subjectMarker gene-
Palavras-chave: dc.subjectqPCR-
Palavras-chave: dc.subjectSewage-
Título: dc.titleDevelopment and evaluation of a colorimetric LAMP based-assay targeting the Bacteroides HF183 marker for tracking sewage pollution in environmental waters-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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