Cryopreservation of canine ovarian tissue by slow freezing and vitrification: Evaluation of follicular morphology and apoptosis rate

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Autor(es): dc.contributorUniversidade de Franca-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversità degli Studi di Milano-
Autor(es): dc.creatorLuizari Stábile, Nicole A.-
Autor(es): dc.creatorOliveira, Frederico Rocha de-
Autor(es): dc.creatorFurtado, Ricardo Andrade-
Autor(es): dc.creatorFelippe, Carolina Barretto M L-
Autor(es): dc.creatorTavares, Mariana Riboli-
Autor(es): dc.creatorMartinelli, Paulo E B-
Autor(es): dc.creatorFonseca-Alves, Carlos Eduardo-
Autor(es): dc.creatorSouza, Fabiana Ferreira de-
Autor(es): dc.creatorColombo, Martina-
Autor(es): dc.creatorLuvoni, Gaia Cecilia-
Autor(es): dc.creatorApparício, Maricy-
Data de aceite: dc.date.accessioned2025-08-21T18:47:41Z-
Data de disponibilização: dc.date.available2025-08-21T18:47:41Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2024.08.032-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304543-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304543-
Descrição: dc.descriptionIn this study, we aimed to evaluate the efficacy of cryopreserving canine ovarian tissue using vitrification and slow freezing methods while investigating potential differences in cryotolerance based on follicular type and cryopreservation technique. Twenty-eight ovaries were collected from 14 anoestrus bitches of various breeds, aged between 2 and 5 years, and undergoing elective ovariohysterectomy. The ovaries were sectioned into small fragments and randomly assigned to three groups: vitrification, slow freezing, and a control group (fresh tissue). Vitrification was performed using cryotubes containing DAP 213 solution (2M DMSO, 1M acetamide, 3M propylene glycol) in two stages, while slow freezing involved cryotubes with 1.5M DMSO solution inserted into a programmable machine. The effects of cryopreservation were evaluated by histology and immunohistochemistry (cleaved caspase-3), to determine the percentage of cells undergoing apoptosis. Histological examination revealed that the slow freezing group exhibited a significantly higher percentage of intact follicles (45.75 %) compared to those subjected to vitrification (38.17 %; P = 0.01). Immunohistochemical evaluation further indicated that 84.21 % of the follicles in the slow freezing group did not express caspase-3, suggesting the absence of apoptosis. Conversely, vitrified samples exhibited significantly more apoptotic cells compared to other groups (P < 0.001). Furthermore, early antral follicles displayed a higher susceptibility to degeneration regardless of the cryopreservation method employed. Nevertheless, when comparing the cryopreserved groups, early antral follicles showed greater degeneration in slow freezing group, while preantral follicles were the most affected in the vitrification group. In conclusion, slow freezing demonstrated superior preservation of viable follicles compared to vitrification and emerged as the preferred technique for cryopreserving canine ovarian tissue. These findings contribute valuable insights into optimizing cryopreservation methods for canine ovarian tissue, potentially benefiting reproductive technologies and fertility preservation in canines.-
Descrição: dc.descriptionUniversidade de Franca-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) FCAV, Campus de Jaboticabal-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) FMVZ Campus de Botucatu-
Descrição: dc.descriptionDipartimento di Medicina Veterinaria e Scienze Animali Università degli Studi di Milano-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) FCAV, Campus de Jaboticabal-
Descrição: dc.descriptionUniversidade Estadual Paulista (UNESP) FMVZ Campus de Botucatu-
Formato: dc.format8-14-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBitches-
Palavras-chave: dc.subjectCaspase-3-
Palavras-chave: dc.subjectGonadal tissue-
Palavras-chave: dc.subjectHistology-
Palavras-chave: dc.subjectImmunohistochemistry-
Título: dc.titleCryopreservation of canine ovarian tissue by slow freezing and vitrification: Evaluation of follicular morphology and apoptosis rate-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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