Feasibility of In Vivo Semen Collection and Description of the Morphology and Ultrastructure of the Spermatozoa of Arapaima gigas (Schinz, 1822)

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)-
Autor(es): dc.contributorInst Fed Tocantins IFTO-
Autor(es): dc.contributorNofima AS-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorTorati, Lucas S.-
Autor(es): dc.creatorLopes, Julia T.-
Autor(es): dc.creatorLima, Adriana F.-
Autor(es): dc.creatorPuvanendran, Velmurugu-
Autor(es): dc.creatorBatlouni, Sergio R.-
Autor(es): dc.creatorGaneco-Kirschnik, Luciana N.-
Data de aceite: dc.date.accessioned2025-08-21T20:40:41Z-
Data de disponibilização: dc.date.available2025-08-21T20:40:41Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/fishes9010024-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304419-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304419-
Descrição: dc.descriptionThe pirarucu, Arapaima gigas, is an emblematic endangered Amazon osteoglossid with high potential for aquaculture development. In this study, we report the in vivo collection of semen from pirarucu for the first-time and a complete description of the spermatozoa ultrastructure using scanning and transmission electron microscopy (SEM and TEM). The spermatozoon of pirarucu is biflagellate with no acrosomal cell, comprising a spherical head (8.26 +/- 2.19 mu m(2)), a short intermediate piece region and two long flagella (68.34 +/- 5.69 mu m). The nucleus is spherical and electron-dense, and at its base, there are two nuclear fossae where the centrioles are found. The anterior part of the flagellum is positioned at the lateral base of the nucleus, forming an intermediate piece. Lateral to the intermediate piece, an abundant number of mitochondria are present and occupy most of the cytoplasmic volume. The two flagella were found with a 9 + 2 axonemal structure (nine peripheral doublets and two central microtubules). The central microtubules are aligned with two side fins formed laterally by the axoneme plasma membrane. Side fins were observed from the anterior region to the posterior end. The results show that the spermatozoa of A. gigas are biflagellate, mitochondria-rich and robust, corroborating the external fertilization characteristic of the species. The novel description of sperm morphology presented herein will contribute to advancing the knowledge of and aiding future research on the reproductive biology of this species.-
Descrição: dc.descriptionEuropean Union-
Descrição: dc.descriptionEMBRAPA Fisheries & Aquaculture, BR-77008900 Palmas, Brazil-
Descrição: dc.descriptionInst Fed Tocantins IFTO, Campus Pedro Afonso, BR-77710000 Pedro Afonso, Brazil-
Descrição: dc.descriptionNofima AS, Ctr Marine Aquaculture & Prod Biol, Muninbakken 9, N-9019 Tromso, Norway-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Ctr Aquicultura UNESP CAUNESP, BR-14884900 Jaboticabal, Brazil-
Descrição: dc.descriptionUniv Estadual Paulista UNESP, Ctr Aquicultura UNESP CAUNESP, BR-14884900 Jaboticabal, Brazil-
Descrição: dc.descriptionEuropean Union: 818173-
Formato: dc.format11-
Idioma: dc.languageen-
Publicador: dc.publisherMdpi-
Relação: dc.relationFishes-
???dc.source???: dc.sourceWeb of Science-
Palavras-chave: dc.subjectbiflagellate spermatozoa-
Palavras-chave: dc.subjectOsteoglossidae-
Palavras-chave: dc.subjectpirarucu-
Palavras-chave: dc.subjectreproduction-
Palavras-chave: dc.subjectultrastructure-
Título: dc.titleFeasibility of In Vivo Semen Collection and Description of the Morphology and Ultrastructure of the Spermatozoa of <i>Arapaima gigas</i> (Schinz, 1822)-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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