Establishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis)

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Autor(es): dc.contributorUniversidade Federal do Pará (UFPA)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInstituto Biologia e Conservação dos Mamíferos Aquáticos da Amazônia (BioMA)-
Autor(es): dc.creatorTavares, Flávia dos Santos-
Autor(es): dc.creatorMartins, Cesar-
Autor(es): dc.creatorDelella, Flávia Karina-
Autor(es): dc.creatorNascimento, Luís Adriano Santos do-
Autor(es): dc.creatorRodrigues, Angélica Lúcia Figueiredo-
Autor(es): dc.creatorMoreira, Sávia-
Autor(es): dc.creatorCardoso, Adauto Lima-
Autor(es): dc.creatorNoronha, Renata Coelho Rodrigues-
Data de aceite: dc.date.accessioned2025-08-21T18:36:29Z-
Data de disponibilização: dc.date.available2025-08-21T18:36:29Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-03-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ani14050686-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304319-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304319-
Descrição: dc.descriptionThe vulnerable status of the Amazon manatee, Trichechus inunguis, indicates the need to seek measures to guarantee its conservation. In this context, the cultivation of cells in vitro is a strategy that should at least guarantee the preservation of their genetic material. Thus, we established for the first time a primary culture of Amazonian manatee fibroblasts (TINsf) from a skin biopsy of a young male. Karyotypic analysis of the 3rd, 7th, and 12th passages confirmed the taxonomic identity of the species T. inunguis (2n = 56/NF = 92) and indicated that this culture presents genomic stability. Gene and protein expression of vimentin at the 13th passage show the predominant presence of fibroblasts in TINsf. To test the cell line’s responsiveness to materials and demonstrate a possible application of this culture, it was exposed to andiroba seed oil (ASO), and its viability and proliferative capacity were evaluated. ASO demonstrated toxic effects at the highest concentrations and longest exposure times tested, reproducing results observed in human cultures, indicating the applicability of TINsf in toxicological and biotechnological studies. After cryopreservation, the TINsf line maintained its proliferative potential, indicating the establishment of a new culture available for future studies.-
Descrição: dc.descriptionLaboratório de Genética e Biologia Celular Centro de Estudos Avançados da Biodiversidade Instituto de Ciências Biológicas Universidade Federal do Pará, PA-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences at Botucatu Sao Paulo State University—UNESP, SP-
Descrição: dc.descriptionLaboratório de Óleos da Amazônia Universidade Federal do Pará, PA-
Descrição: dc.descriptionInstituto Biologia e Conservação dos Mamíferos Aquáticos da Amazônia (BioMA), PA-
Descrição: dc.descriptionDepartment of Structural and Functional Biology Institute of Biosciences at Botucatu Sao Paulo State University—UNESP, SP-
Idioma: dc.languageen-
Relação: dc.relationAnimals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmazon biobank-
Palavras-chave: dc.subjectandiroba-
Palavras-chave: dc.subjectaquatic mammals-
Palavras-chave: dc.subjectconservation-
Palavras-chave: dc.subjectcryopreservation-
Palavras-chave: dc.subjectendangered species-
Palavras-chave: dc.subjectsirenians-
Título: dc.titleEstablishment and Characterization of a Primary Fibroblast Cell Culture from the Amazonian Manatee (Trichechus inunguis)-
Tipo de arquivo: dc.typelivro digital-
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