Dual nanocarrier of chlorhexidine and fluconazole: Physicochemical characterization and effects on microcosm biofilms and oral keratinocytes

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Western São Paulo (UNOESTE)-
Autor(es): dc.contributorGlasgow Caledonian University-
Autor(es): dc.creatorAraujo, Heitor Ceolin-
Autor(es): dc.creatorPessan, Juliano Pelim-
Autor(es): dc.creatorCaldeirão, Anne Caroline Morais-
Autor(es): dc.creatorSampaio, Caio-
Autor(es): dc.creatorOliveira, Marcelo José dos Santos-
Autor(es): dc.creatorSales, Douglas Henrique-
Autor(es): dc.creatorTeixeira, Silvio Rainho-
Autor(es): dc.creatorConstantino, Carlos José Leopoldo-
Autor(es): dc.creatorDelbem, Alberto Carlos Botazzo-
Autor(es): dc.creatorOliveira, Sandra Helena Penha de-
Autor(es): dc.creatorRamage, Gordon-
Autor(es): dc.creatorMonteiro, Douglas Roberto-
Data de aceite: dc.date.accessioned2025-08-21T20:44:59Z-
Data de disponibilização: dc.date.available2025-08-21T20:44:59Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2023.104699-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304304-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304304-
Descrição: dc.descriptionObjectives: This study assembled and characterized a dual nanocarrier of chlorhexidine (CHX) and fluconazole (FLZ), and evaluated its antibiofilm and cytotoxic effects. Methods: CHX and FLZ were added to iron oxide nanoparticles (IONPs) previously coated by chitosan (CS) and characterized by physical-chemical analyses. Biofilms from human saliva supplemented with Candida species were grown (72 h) on glass discs and treated (24 h) with IONPs-CS carrying CHX (at 39, 78, or 156 µg/mL) and FLZ (at 156, 312, or 624 µg/mL) in three growing associations. IONPs and CS alone, and 156 µg/mL CHX + 624 µg/mL FLZ (CHX156-FLZ624) were tested as controls. Next, microbiological analyses were performed. The viability of human oral keratinocytes (NOKsi lineage) was also determined (MTT reduction assay). Data were submitted to ANOVA or Kruskal-Wallis, followed by Fisher's LSD or Tukey's tests (α=0.05). Results: Nanocarriers with spherical-like shape and diameter around 6 nm were assembled, without compromising the crystalline property and stability of IONPs. Nanocarrier at the highest concentrations was the most effective in reducing colony-forming units of Streptococcus mutans, Lactobacillus spp., Candida albicans, and Candida glabrata. The other carriers and CHX156-FLZ624 showed similar antibiofilm effects, and significantly reduced lactic acid production (p<0.001). Also, a dose-dependent cytotoxic effect against oral keratinocytes was observed for the dual nanocarrier. IONPs-CS-CHX-FLZ and CHX-FLZ significantly reduced keratinocyte viability at CHX and FLZ concentrations ≥7.8 and 31.25 µg/mL, respectively (p<0.05). Conclusion: The nanotherapy developed outperformed the effect of the combination CHX-FLZ on microcosm biofilms, without increasing the cytotoxic effect of the antimicrobials administered. Clinical Significance: The dual nanocarrier is a promising topically-applied therapy for the management of oral candidiasis considering that its higher antibiofilm effects allow the use of lower concentrations of antimicrobials than those found in commercial products.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry-
Descrição: dc.descriptionSchool of Dentistry Presidente Prudente University of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Technology and Applied Sciences (FCT) Department of Physics-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Basic Sciences-
Descrição: dc.descriptionSafeguarding Health through Infection Prevention (SHIP) Research Group Research Centre for Health Glasgow Caledonian University-
Descrição: dc.descriptionPostgraduate Program in Health Sciences University of Western São Paulo (UNOESTE)-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Technology and Applied Sciences (FCT) Department of Physics-
Descrição: dc.descriptionSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Basic Sciences-
Descrição: dc.descriptionFAPESP: 2017/24416–2-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBiofilms-
Palavras-chave: dc.subjectCandida-
Palavras-chave: dc.subjectChlorhexidine-
Palavras-chave: dc.subjectCytotoxicity-
Palavras-chave: dc.subjectFluconazole-
Palavras-chave: dc.subjectIron oxide nanoparticles-
Título: dc.titleDual nanocarrier of chlorhexidine and fluconazole: Physicochemical characterization and effects on microcosm biofilms and oral keratinocytes-
Tipo de arquivo: dc.typelivro digital-
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