Bartonella species in dromedaries and ruminants from Lower Shabelle and Benadir regions, Somalia

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal do Paraná (UFPR)-
Autor(es): dc.contributorAbrar University-
Autor(es): dc.contributorand Range-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorSomali Meat Company-
Autor(es): dc.contributorThe University of North Carolina at Charlotte-
Autor(es): dc.creatorOsman, Aamir M.-
Autor(es): dc.creatorHassan-Kadle, Ahmed A.-
Autor(es): dc.creatorDias, Clara Morato-
Autor(es): dc.creatorIbrahim, Abdalla M.-
Autor(es): dc.creatorCollere, Flávia C. M.-
Autor(es): dc.creatorShair, Mohamed A.-
Autor(es): dc.creatorMontiani-Ferreira, Fabiano-
Autor(es): dc.creatorAndré, Marcos R.-
Autor(es): dc.creatorYusuf, Abdulkarim A.-
Autor(es): dc.creatorVieira, Thállitha S. W. J.-
Autor(es): dc.creatorMachado, Rosangela Z.-
Autor(es): dc.creatorVieira, Rafael F. C.-
Data de aceite: dc.date.accessioned2025-08-21T23:30:13Z-
Data de disponibilização: dc.date.available2025-08-21T23:30:13Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1111/zph.13158-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304247-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304247-
Descrição: dc.descriptionBackground: Bartonellosis, caused by bacteria of the genus Bartonella, is a zoonotic disease with several mammalian reservoir hosts. In Somalia, a country heavily reliant on livestock, zoonotic diseases pose significant public health and economic challenges. To the best of our knowledge, no study has been performed aiming to verify the occurrence of Bartonella spp. in Somalia. This study investigated the occurrence and molecular characterization of Bartonella in dromedary (Camelus dromedarius, Linnaeus, 1758), cattle, sheep, and goats from Somalia. Materials and Methods: 530 blood samples were collected from various animals (155 dromedary, 199 goat, 131 cattle, and 45 sheep) in Benadir and Lower Shabelle regions. DNA was extracted for molecular analysis, and a qPCR assay targeting the NADH dehydrogenase gamma subunit (nuoG) gene was used for Bartonella screening. Positive samples were also subjected to PCR assays targeting seven molecular markers including: nuoG, citrate synthase gene (gltA), RNA polymerase beta-subunit gene (rpoB), riboflavin synthase gene (ribC), 60 kDa heat-shock protein gene (groEL), cell division protein gene (ftsZ), and pap31 and qPCR targeting the 16-23S rRNA internal transcribed spacer (ITS) followed by Sanger sequencing, BLASTn and phylogenetic analysis. Results: Out of 530 tested animals, 5.1% were positive for Bartonella spp. by the nuoG qPCR assay. Goats showed the highest Bartonella occurrence (17/199, 8.5%), followed by sheep (6/44, 6.8%), cattle (4/131, 3.1%), and dromedary (1/155, 1.9%). Goats, sheep, and cattle had higher odds of infection compared to dromedary. Among nuoG qPCR-positive samples, 11.1%, 14.8%, 11.1%, and 25.9% were positive in PCR assays based on nuoG, gltA, and pap31 genes, and in the qPCR based on the ITS region, respectively. On the other hand, nuoG qPCR-positive samples were negative in the PCR assays targeting the ribC, rpoB, ftsZ, and groEL genes. While Bartonella bovis sequences were detected in cattle (nuoG and ITS) and goats (gltA), Bartonella henselae ITS sequences were detected in dromedary, goat, and sheep. Phylogenetic analysis placed gltA Bartonella sequence from a goat in the same clade of B. bovis. Conclusion: The present study showed, for the first time, molecular evidence of Bartonella spp. in dromedary and ruminants from Somalia and B. henselae in sheep and goats globally. These findings contribute valuable insights into Bartonella spp. occurrence in Somali livestock, highlighting the need for comprehensive surveillance and control measures under the One Health approach.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionUniversidade Federal do Paraná-
Descrição: dc.descriptionGraduate Program on Veterinary Sciences Universidade Federal do Paraná, Paraná-
Descrição: dc.descriptionSomali One Health Centre Abrar University-
Descrição: dc.descriptionDepartment of Animal Health and Veterinary Services Ministry of Livestock Forestry and Range-
Descrição: dc.descriptionAbrar Research and Training Centre Abrar University-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health Faculty of Agrarian and Veterinary Sciences São Paulo State University (FCAV/UNESP)-
Descrição: dc.descriptionDepartment of Veterinary Medicine Universidade Federal do Paraná, Paraná-
Descrição: dc.descriptionDepartment of Slaughterhouses Somali Meat Company-
Descrição: dc.descriptionCenter for Computational Intelligence to Predict Health and Environmental Risks (CIPHER) The University of North Carolina at Charlotte-
Descrição: dc.descriptionDepartment of Epidemiology and Community Health The University of North Carolina at Charlotte-
Descrição: dc.descriptionVector-Borne Bioagents Laboratory (VBBL) Department of Pathology Reproduction and One Health Faculty of Agrarian and Veterinary Sciences São Paulo State University (FCAV/UNESP)-
Formato: dc.format568-577-
Idioma: dc.languageen-
Relação: dc.relationZoonoses and Public Health-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBartonella bovis-
Palavras-chave: dc.subjectBartonella henselae-
Palavras-chave: dc.subjectcamels-
Palavras-chave: dc.subjectruminants-
Palavras-chave: dc.subjectsub-Saharan Africa-
Palavras-chave: dc.subjectzoonotic diseases-
Título: dc.titleBartonella species in dromedaries and ruminants from Lower Shabelle and Benadir regions, Somalia-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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