Exploring the efficacy of in-vitro low-temperature plasma treatment on single and multispecies dental cariogenic biofilms

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorLake Erie College of Osteopathic Medicine-
Autor(es): dc.contributorUniversity at Buffalo School of Dental Medicine-
Autor(es): dc.creatorFigueira, Leandro Wagner-
Autor(es): dc.creatorPanariello, Beatriz-
Autor(es): dc.creatorKoga-Ito, Cristiane Y.-
Autor(es): dc.creatorDuarte, Simone-
Data de aceite: dc.date.accessioned2025-08-21T19:45:18Z-
Data de disponibilização: dc.date.available2025-08-21T19:45:18Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1038/s41598-024-70943-0-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304235-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304235-
Descrição: dc.descriptionThe primary aim of this study was to investigate the impact of treatment with low-temperature plasma (LTP) for varying exposure durations on a multispecies cariogenic biofilm comprising C. albicans, L. casei, and S. mutans, as well as on single-species biofilms of L. casei and C. albicans, cultured on hydroxyapatite discs. Biofilms were treated with LTP-argon at a 10 mm distance for 30 s, 60 s, and 120 s. Chlorhexidine solution (0.12%) and NaCl (0.89%) were used as positive (PC) and negative controls (NC), respectively. Argon flow only was also used as gas flow control (F). Colony-forming units (CFU) recovery and confocal laser scanning microscopy (CLSM) were used to analyze biofilm viability. LTP starting at 30 s of application significantly reduced the viability of multispecies biofilms by more than 2 log10 in all treated samples (p < 0.0001). For single-species biofilms, L. casei showed a significant reduction compared to PC and NC of over 1 log10 at all exposure times (p < 0.0001). In the case of C. albicans biofilms, LTP treatment compared to PC and NC resulted in a significant decrease in bacterial counts when applied for 60 and 120 s (1.55 and 1.90 log10 CFU/mL, respectively) (p < 0.0001). A significant effect (p ≤ 0.05) of LTP in single-species biofilms was observed to start at 60 s of LTP application compared to F, suggesting a time-dependent effect of LTP for the single-species biofilms of C. albicans and L. casei. LTP is a potential mechanism in treating dental caries by being an effective anti-biofilm therapy of both single and multispecies cariogenic biofilms.-
Descrição: dc.descriptionDepartment of Environmental Engineering and Oral Biopathology Graduate Program Institute of Science and Technology São Paulo State University UNESP, São José dos Campos-
Descrição: dc.descriptionSchool of Dental Medicine Lake Erie College of Osteopathic Medicine-
Descrição: dc.descriptionDepartment of Restorative Dentistry University at Buffalo School of Dental Medicine-
Descrição: dc.descriptionDepartment of Environmental Engineering and Oral Biopathology Graduate Program Institute of Science and Technology São Paulo State University UNESP, São José dos Campos-
Idioma: dc.languageen-
Relação: dc.relationScientific Reports-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibacterial-
Palavras-chave: dc.subjectDental caries-
Palavras-chave: dc.subjectLow-temperature plasma-
Palavras-chave: dc.subjectOral biofilms-
Título: dc.titleExploring the efficacy of in-vitro low-temperature plasma treatment on single and multispecies dental cariogenic biofilms-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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