Hyperglycemia influences the cell proliferation and death of the rat endocrine pancreas in the neonatal period

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Mato Grosso (UFMT)-
Autor(es): dc.creatorBarco, Vinícius S.-
Autor(es): dc.creatorGallego, Franciane Q.-
Autor(es): dc.creatorMiranda, Carolina A.-
Autor(es): dc.creatorSouza, Maysa R.-
Autor(es): dc.creatorVolpato, Gustavo T.-
Autor(es): dc.creatorDamasceno, Débora C.-
Data de aceite: dc.date.accessioned2025-08-21T18:02:36Z-
Data de disponibilização: dc.date.available2025-08-21T18:02:36Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-08-15-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2024.122854-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304222-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304222-
Descrição: dc.descriptionAims: To evaluate the cell proliferation and death, and structural morphology of the pancreatic islet cells of the rats with hyperglycemia in the first month of life and compare to those of the control rats. Main methods: Female Sprague-Dawley newborn rats received Streptozotocin (a beta-cytotoxic drug) at birth for diabetes induction. Control and hyperglycemic animals were euthanized on different days of life: 5, 10, 15, and 30. The pancreas was collected and processed for immunohistochemical analysis of cleaved Caspase-3 (cell death), Ki-67 (cell proliferation), PDX-1 (transcription factor responsible for insulin synthesis), and endocrine hormones (insulin, glucagon, and somatostatin). Key findings: Control females showed a higher percentage (%) of Ki-67-positive(+) cells on D10 and D15, a higher % of insulin+ and somatostatin+ cells on D15 and D30, a lower % of PDX-1+ cells on D10, and a higher % of glucagon+ cells on D10 and D30. Hyperglycemic females showed a lower % of Ki-67+ cells on D15, a higher % of cleaved Caspase-3+ cells on D15, and insulin+ cells on D15 and D30. In the comparison among the experimental groups, the hyperglycemic females showed an increased % of cleaved Caspase-3+ and Ki-67+ cells and a lower % of PDX-1+ cells. Significance: This study enabled a better understanding of the abnormal pancreas development regarding cellular proliferation, apoptosis, and hormonal synthesis in the neonatal period. Thus, the pancreatic islets of hyperglycemic rats do not reestablish the normal endocrine cell population, and cellular apoptosis overcame the proliferative activity of these cells.-
Descrição: dc.descriptionLaboratory of Experimental Research on Gynecology and Obstetrics of UNIPEX Postgraduate Course on Tocogynecology Botucatu Medical School Sao Paulo State University (Unesp), Sao Paulo State-
Descrição: dc.descriptionLaboratory of System Physiology and Reproductive Toxicology Institute of Biological and Health Sciences Federal University of Mato Grosso (UFMT), Mato Grosso State-
Descrição: dc.descriptionLaboratory of Experimental Research on Gynecology and Obstetrics of UNIPEX Postgraduate Course on Tocogynecology Botucatu Medical School Sao Paulo State University (Unesp), Sao Paulo State-
Idioma: dc.languageen-
Relação: dc.relationLife Sciences-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectIslet cells-
Palavras-chave: dc.subjectMurines-
Palavras-chave: dc.subjectNewborn-
Palavras-chave: dc.subjectPancreas-
Palavras-chave: dc.subjectRemodeling-
Título: dc.titleHyperglycemia influences the cell proliferation and death of the rat endocrine pancreas in the neonatal period-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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