In Vitro Evaluation of Sodium Hypochlorite, Chlorhexidine, Propolis, and Calcium Hydroxide Effect on Lipoteichoic-Acid-Induced Proinflammatory Cytokines Production

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidad Espíritu Santo-
Autor(es): dc.creatorde Oliveira, Luciane Dias-
Autor(es): dc.creatorde Carvalho, Lara Steffany-
Autor(es): dc.creatorXavier, Ana Claudia Carvalho-
Autor(es): dc.creatorde Oliveira, Felipe Eduardo-
Autor(es): dc.creatorLeão, Mariella Vieira Pereira-
Autor(es): dc.creatorDiamantino, Mariana Gadelho Gimenez-
Autor(es): dc.creatorKhoury, Rayana Duarte-
Autor(es): dc.creatorValera, Marcia Carneiro-
Autor(es): dc.creatorCarvalho, Cláudio Antonio Talge-
Autor(es): dc.creatorAbu Hasna, Amjad-
Data de aceite: dc.date.accessioned2025-08-21T21:45:44Z-
Data de disponibilização: dc.date.available2025-08-21T21:45:44Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/dj12090286-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304180-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304180-
Descrição: dc.descriptionThis study aimed to evaluate the effects of sodium hypochlorite (NaOCl), chlorhexidine (CHX), and the glycolic extract of propolis (GEP) as endodontic irrigants and of calcium hydroxide [Ca(OH)2], CHX, or Ca(OH)2 + CHX as intracanal medications on the capacity of the lipoteichoic acid (LTA) of Enterococcus faecalis in macrophages’ proinflammatory cytokines production. Freshly extracted 108 human single-rooted teeth were used in this study. The LTA of E. faecalis was standardized in double-distilled pyrogen-free water (250 µg/mL) and inoculated into the specimens subdivided into nine subgroups (n = 12). Cultures of murine macrophages (RAW 264.7) were treated with 30 µL of each sample collected from root canals and incubated (37 °C, 5% CO2) for 24 h. Lastly, anti-TNF-α, anti-IL-6, anti-IP-10, anti-MIP-1α, anti-G-CSF, and anti-IL-1β DuoSet kits were used to perform an ELISA assay. Data were analyzed using one-way ANOVA and Tukey test (p ≥ 0.05). It was found that 1% NaOCl was the most effective irrigant in reducing the capacity of LTA in cytokines production, followed by 12% GEP and 2% CHX, respectively. Ca(OH)2 + CHX presented the best results when associated with NaOCl or GEP. Thus, NaOCl or GEP associated with Ca(OH)2 + CHX were effective in reducing the capacity of LTA in different macrophages pro-inflammatory cytokines production.-
Descrição: dc.descriptionDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University, Campus of São José dos Campos-
Descrição: dc.descriptionDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University, Campus of São José dos Campos-
Descrição: dc.descriptionSchool of Dentistry Universidad Espíritu Santo-
Descrição: dc.descriptionDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology São Paulo State University, Campus of São José dos Campos-
Descrição: dc.descriptionDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University, Campus of São José dos Campos-
Idioma: dc.languageen-
Relação: dc.relationDentistry Journal-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcalcium hydroxide-
Palavras-chave: dc.subjectchlorhexidine-
Palavras-chave: dc.subjectcytokines-
Palavras-chave: dc.subjectEnterococcus faecalis-
Palavras-chave: dc.subjectlipoteichoic acid-
Palavras-chave: dc.subjectpropolis-
Palavras-chave: dc.subjectsodium hypochlorite-
Título: dc.titleIn Vitro Evaluation of Sodium Hypochlorite, Chlorhexidine, Propolis, and Calcium Hydroxide Effect on Lipoteichoic-Acid-Induced Proinflammatory Cytokines Production-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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