Antibacterial, cytotoxic and mechanical properties of a orthodontic cement with phosphate nano-sized and phosphorylated chitosan: An in vitro study

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversity of Ribeirão Preto - UNAERP-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorFernandes, Gabriela Leal Peres-
Autor(es): dc.creatorVanim, Manuela Marquesini-
Autor(es): dc.creatorDelbem, Alberto Carlos Botazzo-
Autor(es): dc.creatorMartorano, Antônio Secco-
Autor(es): dc.creatorRaucci, Larissa Moreira Spinola de Castro-
Autor(es): dc.creatorde Oliveira, Paulo Tambasco-
Autor(es): dc.creatorZucolotto, Valtencir-
Autor(es): dc.creatorDias, Bruna Juliana Moreira-
Autor(es): dc.creatorBrighenti, Fernanda Lourenção-
Autor(es): dc.creatorde Oliveira, Analú Barros-
Autor(es): dc.creatorMoraes, João Carlos Silos-
Autor(es): dc.creatorde Camargo, Emerson Rodrigues-
Autor(es): dc.creatorDanelon, Marcelle-
Data de aceite: dc.date.accessioned2025-08-21T23:07:26Z-
Data de disponibilização: dc.date.available2025-08-21T23:07:26Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-07-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jdent.2024.105073-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304170-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304170-
Descrição: dc.descriptionObjectives: Evaluate, in vitro, the effect of incorporating nano-sized sodium trimetaphosphate (TMPnano) and phosphorylated chitosan (Chi-Ph) into resin-modified glass ionomer cement (RMGIC) used for orthodontic bracket cementation, on mechanical, fluoride release, antimicrobial and cytotoxic properties. Methods: RMGIC was combined with Chi-Ph (0.25%/0.5%) and/or TMPnano (14%). The diametral compressive/tensile strength (DCS/TS), surface hardness (SH) and degree of conversion (%DC) were determined. For fluoride (F) release, samples were immersed in des/remineralizing solutions. Antimicrobial/antibiofilm activity was evaluated by the agar diffusion test and biofilm metabolism (XTT). Cytotoxicity in fibroblasts was assessed with the resazurin method. Results: After 24 h, the RMGIC-14%TMPnano group showed a lower TS value (p < 0.001); after 7 days the RMGIC-14%TMPnano-0.25%Chi-Ph group showed the highest value (p < 0.001). For DCS, the RMGIC group (24 h) showed the highest value (p < 0.001); after 7 days, the highest value was observed for the RMGIC-14%TMPnano-0.25%Chi-Ph (p < 0.001). RMGIC-14%TMPnano, RMGIC-14%TMPnano-0.25%Chi-Ph, RMGIC-14%TMPnano-0.5%Chi-Ph showed higher and similar release of F (p > 0.001). In the SH, the RMGIC-0.25%Chi-Ph; RMGIC-0.5%Chi-Ph; RMGIC-14%TMPnano-0.5%Chi-Ph groups showed similar results after 7 days (p > 0.001). The RMGIC-14%TMPnano-0.25%Chi-Ph group showed a better effect on microbial/antibiofilm growth, and the highest efficacy on cell viability (p < 0.001). After 72 h, only the RMGIC-14%TMPnano-0.25%Chi-Ph group showed cell viability (p < 0.001). Conclusion: The RMGIC-14%TMPnano-0.25%Chi-Ph did not alter the physical-mechanical properties, was not toxic to fibroblasts and reduced the viability and metabolism of S. mutans. Clinical relevance: The addition of phosphorylated chitosan and organic phosphate to RMGIC could provide an antibiofilm and remineralizing effect on the tooth enamel of orthodontic patients, who are prone to a high cariogenic challenge due to fluctuations in oral pH and progression of carious lesions.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Rua José Bonifácio 1193, SP-
Descrição: dc.descriptionSchool of Dentistry University of Ribeirão Preto - UNAERP, SP-
Descrição: dc.descriptionSchool of Dentistry of Ribeirão Preto - University of São Paulo, SP-
Descrição: dc.descriptionSão Carlos Institute of Physics University of São Paulo, SP-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University (Unesp) School of Dentistry, R. Humaitá, 1680 - Centro, São Paulo-
Descrição: dc.descriptionDepartment of Physics and Chemistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionInterdisciplinary Laboratory of Electrochemistry and Ceramics Department of Chemistry Federal University of São Carlos (UFSCar), Rod. Washington Luiz, s/n, São Carlos-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, Rua José Bonifácio 1193, SP-
Descrição: dc.descriptionDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University (Unesp) School of Dentistry, R. Humaitá, 1680 - Centro, São Paulo-
Descrição: dc.descriptionDepartment of Physics and Chemistry São Paulo State University (UNESP), SP-
Descrição: dc.descriptionFAPESP: #2021/05532-7-
Descrição: dc.descriptionFAPESP: #2021/14835-3-
Idioma: dc.languageen-
Relação: dc.relationJournal of Dentistry-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectChitosan-
Palavras-chave: dc.subjectCytotoxicity-
Palavras-chave: dc.subjectMicrobiota-
Palavras-chave: dc.subjectOrthodontics-
Palavras-chave: dc.subjectPhosphates-
Título: dc.titleAntibacterial, cytotoxic and mechanical properties of a orthodontic cement with phosphate nano-sized and phosphorylated chitosan: An in vitro study-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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