Intratesticular transplantation of allogenic mesenchymal stem cells mitigates testicular destruction after induced heat stress in Miniature-horse stallions

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRoss University School of Veterinary Medicine-
Autor(es): dc.creatorPapa, Patricia M.-
Autor(es): dc.creatorSegabinazzi, Lorenzo G.T.M.-
Autor(es): dc.creatorFonseca-Alves, Carlos E.-
Autor(es): dc.creatorPapa, Frederico O.-
Autor(es): dc.creatorAlvarenga, Marco A.-
Data de aceite: dc.date.accessioned2025-08-21T15:32:31Z-
Data de disponibilização: dc.date.available2025-08-21T15:32:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jevs.2023.104961-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304160-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304160-
Descrição: dc.descriptionTesticular degeneration (TD) is the most frequent cause of sub or infertility in stallions. Currently, mesenchymal stem cells (MSC) have been studied as a therapeutic option for several diseases including induced-TD in laboratory animals. Therefore, this study aimed to evaluate the effect of intratesticular MSC therapy on the testicular histology of stallions submitted to scrotal heat stress. Ten healthy Miniature-horse stallions were submitted to testicular heat stress induced by a heating wrap device (42-45°C). Afterward, the stallions were divided into two groups and treated seven days later. MSCs-treated stallions were treated with an intratesticular injection of 10 × 106 of MSCs diluted in 5 mL of PBS, whereas placebo-treated stallions had 5 mL of PBS intratesticular injected. All stallions had testicular biopsies collected seven days before and one- and 14-days post-heat stress and were castrated 30 days after testicular insult. Tissue sections were stained with H&E and evaluated for the tubular and luminal diameter, epithelial thickness, seminiferous tubules (STs) integrity, the number of spermatozoa in the STs, and the percent of abnormal STs. Significance was set at P≤0.05. In both groups, testicular heat stress damaged the STs (P<0.05). However, STs’ parameters were improved in MSCs-treated stallions compared to placebo-treated stallions 30 days after the testicular insult (P<0.05). In conclusion, the results of the present study suggest that intratesticular MSC therapy provided a therapeutic advantage in rescuing acute TD in stallions. However, further studies are essential to evaluate the benefits of this therapy on semen parameters and stallions with idiopathic TD.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP-
Descrição: dc.descriptionDepartment of Clinical Sciences Ross University School of Veterinary Medicine, St. Kitts, West Indies-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science FMVZ Universidade Estadual Paulista “Júlio de Mesquita Filho” UNESP-
Idioma: dc.languageen-
Relação: dc.relationJournal of Equine Veterinary Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectCell therapy-
Palavras-chave: dc.subjectSemen-
Palavras-chave: dc.subjectSeminiferous tubules-
Palavras-chave: dc.subjectTesticular degeneration-
Título: dc.titleIntratesticular transplantation of allogenic mesenchymal stem cells mitigates testicular destruction after induced heat stress in Miniature-horse stallions-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.