Monitoring the Intracellular Fate of Molecular Beacons: The Challenge of False Positive Signals

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Autor(es): dc.contributorLund University-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidad de Chile-
Autor(es): dc.contributorScania University Hospital-
Autor(es): dc.creatorVolpati, Diogo-
Autor(es): dc.creatorAoki, Pedro H. B.-
Autor(es): dc.creatorJohansson, Therese B.-
Autor(es): dc.creatorMunita, Roberto-
Autor(es): dc.creatorEkstrand, Frida-
Autor(es): dc.creatorRuhrmann, Sabrina-
Autor(es): dc.creatorBacos, Karl-
Autor(es): dc.creatorLing, Charlotte-
Autor(es): dc.creatorPrinz, Christelle N.-
Data de aceite: dc.date.accessioned2025-08-21T19:41:59Z-
Data de disponibilização: dc.date.available2025-08-21T19:41:59Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-05-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/anbr.202300147-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304142-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304142-
Descrição: dc.descriptionMolecular beacons (MBs) have been used on surfaces for detecting oligonucleotides. Attempts to use them intracellularly for monitoring mRNA content have been made, however, without any clear conclusion regarding the reliability of the method, mainly due to false positive signals. To reach an understanding of the intracellular fate of MBs, a critical question remains: how long after MB delivery and where in the cell does a false positive signal appear? To answer that question, the MB delivery method should allow for a time-stamped synchronized delivery of MBs to multiple cells, resulting in MBs being distributed in the cytosol immediately after delivery. Herein, nanostraws are used to inject MBs targeting insulin (Ins1) mRNA directly in the cytosol of clonal beta-cells, and the evolution of the MB fluorescence in time and space is monitored. The results show an MB translocation to the nucleus, where MBs are degraded or where they open nonspecifically, before the fluorophore alone is expelled back from the nucleus to the cytosol. The signal translocation to the nucleus and back to the cytosol is faster when scrambled MBs are used. The results shed light on the intracellular fate of MBs and highlight the short time scales before false positive signals become predominant.-
Descrição: dc.descriptionVetenskapsrådet-
Descrição: dc.descriptionEuropean Research Council-
Descrição: dc.descriptionStiftelsen för Strategisk Forskning-
Descrição: dc.descriptionDivision of Solid State Physics and NanoLund Lund University-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages, SP-
Descrição: dc.descriptionDivision of Molecular Hematology Department of Laboratory Medicine Lund University-
Descrição: dc.descriptionAdvanced Center for Chronic Diseases (ACCDiS) Department of Biochemistry and Molecular Biology Faculty of Chemical and Pharmaceutical Sciences Universidad de Chile-
Descrição: dc.descriptionEpigenetics and Diabetes Unit Lund University Diabetes Centre Department of Clinical Sciences Malmö Scania University Hospital-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Sciences Humanities and Languages, SP-
Descrição: dc.descriptionVetenskapsrådet: 2018-02435-
Descrição: dc.descriptionVetenskapsrådet: 2019-03472-
Descrição: dc.descriptionVetenskapsrådet: 2021-00628-
Descrição: dc.descriptionEuropean Research Council: 662206-
Descrição: dc.descriptionStiftelsen för Strategisk Forskning: IRC15-0067-
Descrição: dc.descriptionStiftelsen för Strategisk Forskning: ITM17-0275-
Idioma: dc.languageen-
Relação: dc.relationAdvanced NanoBiomed Research-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectbeta-cells-
Palavras-chave: dc.subjectcell interrogation-
Palavras-chave: dc.subjectlive cell imaging-
Palavras-chave: dc.subjectmolecular beacons-
Palavras-chave: dc.subjectnanostraws-
Título: dc.titleMonitoring the Intracellular Fate of Molecular Beacons: The Challenge of False Positive Signals-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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