Possible role of annexin A1/FPR2 pathway in COX2/NLRP3 inflammasome regulation in alveolar bone cells of estrogen-deficient female rats with diabetes mellitus

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorSasso, Gisela Rodrigues Da Silva-
Autor(es): dc.creatorCerri, Paulo Sérgio-
Autor(es): dc.creatorSasso-Cerri, Estela-
Autor(es): dc.creatorSimões, Manuel Jesus-
Autor(es): dc.creatorGil, Cristiane Damas-
Autor(es): dc.creatorFlorencio-Silva, Rinaldo-
Data de aceite: dc.date.accessioned2025-08-21T20:52:30Z-
Data de disponibilização: dc.date.available2025-08-21T20:52:30Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1002/JPER.23-0530-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304078-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304078-
Descrição: dc.descriptionBackground: Annexin A1 (ANXA1) and the NOD-like receptor family pyrin domain-containing protein 3 (NLRP3) inflammasome play important roles in bone remodeling. However, expression profiles of these factors in bone cells under diabetes mellitus (DM) and estrogen-deficient conditions are poorly understood. This study investigated the immunoexpression of ANXA1 and its formyl peptide receptor 2 (FPR2), as well as NLRP3 inflammasome mediators, during remodeling of the alveolar process in diabetic and estrogen-deficient rats. Methods: Twenty adult female Wistar rats were divided into four groups (n = 5): Sham-operated (SHAM) and ovariectomized (OVX) rats received a vehicle solution, and SHAM and OVX rats were intraperitoneally administered 60 mg/kg/body weight (BW) of streptozotocin (STZ) to induce DM (SHAM-Di and OVX-Di groups). After 7 weeks, the rats were euthanized and their maxillae were fixed in phosphate-buffered 4% formaldehyde and embedded in paraffin. Sections were stained with hematoxylin/eosin (H&E) and picrosirius red or subjected to immunohistochemical detection of ANXA1, FPR2, NLRP3, interleukin-1β (IL-1β), and cyclooxygenase-2 (COX2). Results: Estrogen deficiency and DM were associated with deleterious effects in bone tissue, as evidenced by a lower number of osteocytes and higher number of empty lacunae in the SHAM-Di and OVX-Di groups compared to the nondiabetic groups. Both diabetic groups showed a smaller vascular area and weaker collagen fiber birefringence intensity in alveolar bone tissue. A significantly higher number of ANXA1/FPR2-positive osteoblasts, osteocytes, and osteoclasts was accompanied by a significantly higher number of these cells immunolabeled for COX2, NLRP3, and IL-1β in the diabetic and OVX groups, especially in both estrogen-deficient and diabetic rats. Conclusion: These results indicate a possible role for the ANXA1/FPR2 pathway as a fine-tuning/anti-inflammatory regulator to counterbalance exacerbated COX2/NLRP3/IL-1β activation in bone cells during bone remodeling under estrogen deficiency and DM.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Morphology and Genetics Laboratory of Histology and Structural Biology Federal University of São Paulo – Paulista School of Medicine (UNIFESP – EPM), SP-
Descrição: dc.descriptionSchool of Dentistry Araraquara – Department of Morphology Genetics Orthodontics and Pediatric Dentistry – Laboratory of Histology and Embryology São Paulo State University (UNESP), SP-
Descrição: dc.descriptionSchool of Dentistry Araraquara – Department of Morphology Genetics Orthodontics and Pediatric Dentistry – Laboratory of Histology and Embryology São Paulo State University (UNESP), SP-
Formato: dc.format749-763-
Idioma: dc.languageen-
Relação: dc.relationJournal of Periodontology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectalveolar process-
Palavras-chave: dc.subjectannexin A1-
Palavras-chave: dc.subjectdiabetes mellitus-
Palavras-chave: dc.subjectinflammasome-
Palavras-chave: dc.subjectovariectomy-
Título: dc.titlePossible role of annexin A1/FPR2 pathway in COX2/NLRP3 inflammasome regulation in alveolar bone cells of estrogen-deficient female rats with diabetes mellitus-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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