Biosensors for amplification-free viral RNA detection

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorParassol, Brenda G.-
Autor(es): dc.creatorKusimoto Takeuti, Nayla Naomi-
Autor(es): dc.creatorFaria, Henrique Antonio Mendonça-
Autor(es): dc.creatorJorge, Kelly C.-
Autor(es): dc.creatorSampaio, Isabella-
Autor(es): dc.creatorZucolotto, Valtencir-
Autor(es): dc.creatorVieira, Nirton C.S.-
Data de aceite: dc.date.accessioned2025-08-21T19:30:53Z-
Data de disponibilização: dc.date.available2025-08-21T19:30:53Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.biosx.2024.100478-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/304031-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/304031-
Descrição: dc.descriptionViruses are infectious agents that cause various diseases worldwide. The recent COVID-19 pandemic has shown the need for rapid and reliable tests to confirm viral infections, aiming at the rapid isolation, treatment, and identification of high-incidence regions. Rapid antigen tests based on lateral flow immunochromatography have proven to be very useful. However, they are not accurate in patients with low viral loadings. The gold standard test is RT-PCR, which identifies parts of the viral genome by detecting specific DNA or RNA sequences. RT-PCR or similar tests such as RT-LAMP involve several steps for sample preparation and amplification of target sequences, require trained personnel to be performed, and can be time-consuming and expensive, limiting their point-of-care application. Biosensors are promising analytical devices for detecting nucleic acids, mainly RNA from viruses, offering advantages such as rapid results, high sensitivity, and low cost compared with the RT-PCR test since the amplification of target sequences is not necessary. Recently, several biosensors have been developed to detect RNA viruses without sequence amplification. Here, we present a review on the design and technology of amplification-free biosensors for the detection of viral RNA as an alternative for diagnosing infectious diseases. The challenges and advances for the point-of-care electrochemical, electrical, and optical biosensors will be addressed.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionInstitute of Science and Technology Federal University of São Paulo, SP-
Descrição: dc.descriptionNanomedicine and Nanotoxicology Group Physics Institute of São Carlos University of São Paulo, CP 369-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) Institute of Chemistry, SP-
Descrição: dc.descriptionCNPq: 309943/2020-5-
Descrição: dc.descriptionCNPq: 408339/2023-3-
Idioma: dc.languageen-
Relação: dc.relationBiosensors and Bioelectronics: X-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAmplification-free-
Palavras-chave: dc.subjectBiosensors-
Palavras-chave: dc.subjectDiagnosis-
Palavras-chave: dc.subjectRNA-
Palavras-chave: dc.subjectViruses-
Título: dc.titleBiosensors for amplification-free viral RNA detection-
Tipo de arquivo: dc.typevídeo-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.