Detection and genomic characterization of a multidrug-resistant Salmonella Newport co-harbouring blaCMY-2, qnrB19 and mcr-9 from the diarrheic faeces of a foal

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorInstituto Adolfo Lutz-
Autor(es): dc.creatorBraga, Pollyana Rennó Campos-
Autor(es): dc.creatordos Santos, Carla Adriana-
Autor(es): dc.creatorBertani, Amanda Maria de Jesus-
Autor(es): dc.creatorVieira, Thais-
Autor(es): dc.creatorAmarante, Ariadne Ferreira-
Autor(es): dc.creatorReis, Alex Domingos-
Autor(es): dc.creatorSacchi, Cláudio Tavares-
Autor(es): dc.creatorCamargo, Carlos Henrique-
Autor(es): dc.creatorRibeiro, Marcio Garcia-
Autor(es): dc.creatorBorges, Alexandre Secorun-
Autor(es): dc.creatorTiba-Casas, Monique Ribeiro-
Data de aceite: dc.date.accessioned2025-08-21T23:25:55Z-
Data de disponibilização: dc.date.available2025-08-21T23:25:55Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jgar.2023.09.019-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/303997-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/303997-
Descrição: dc.descriptionObjectives: This study reports the genomic characterization of the multidrug resistant Salmonella Newport strain 195_20 recovered from the diarrheic faeces of a foal in Brazil and co-harbouring the mcr-9, blaCMY-2 and qnrB19 antibiotic resistance genes. Methods: Bacterial isolate positive for mobile colistin resistance gene (mcr-9) was submitted to antimicrobial susceptibility testing by disk diffusion and broth microdilution for colistin and polymyxin B. The isolate was submitted to whole genome sequencing by Illumina technology and Nanopore Sequencing. Conjugation assays, plasmid sizes determined by S1-PFGE and plasmid content were investigated by hybrid assembly after MinIon long reads sequencing. Results: Isolate 195_20 was identified as sequence type ST45, resistant to penicillin and cephalosporins (ampicillin, ceftazidime, ceftriaxone and cefotaxime), aminoglycosides (streptomycin and gentamicin), phenicol (chloramphenicol), quinolones and fluoroquinolones (nalidixic acid, ciprofloxacin, and pefloxacin), folate pathway antagonists (sulfonamides and trimethoprim-sulfamethoxazole), and tetracycline. A transferable IncHI2/IncHI2A plasmid sized ca. 262kb was found to carry the mcr-9 gene in a module consisting of IS903-mcr-9-wbuC-IS26. In addition, an 174kb IncC and a 48kb IncN plasmid were also identified in the 195_20 isolate, carrying blaCMY-2 and qnrB19, respectively. Conclusions: Not surprisingly, isolate 195_20 was susceptible to polymyxins, possibly due to absence of qseBC regulatory operon. Presence of mobile colistin resistance (mcr-9), third-generation cephalosporins (blaCMY-2) and quinolone (qnrB19) resistance determinants in zoonotic pathogens from animals in close contact with humans alerts for the possible route of transmission between these different reservoirs.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionDepartment of Veterinary Clinical Science School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (Unesp), SP-
Descrição: dc.descriptionInstituto Adolfo Lutz, SP-
Descrição: dc.descriptionDepartment of Veterinary Clinical Science School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (Unesp), SP-
Descrição: dc.descriptionFAPESP: 2017/50333-7-
Descrição: dc.descriptionFAPESP: 2021/12219-3-
Formato: dc.format198-201-
Idioma: dc.languageen-
Relação: dc.relationJournal of Global Antimicrobial Resistance-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectColistin-
Palavras-chave: dc.subjectIllumina sequencing-
Palavras-chave: dc.subjectNanopore sequencing-
Palavras-chave: dc.subjectPlasmids-
Palavras-chave: dc.subjectSalmonella Newport-
Título: dc.titleDetection and genomic characterization of a multidrug-resistant Salmonella Newport co-harbouring blaCMY-2, qnrB19 and mcr-9 from the diarrheic faeces of a foal-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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