Detection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorIrvine (UCI)-
Autor(es): dc.contributorAraçatuba Regional Laboratory-
Autor(es): dc.contributorBauru II Regional Laboratory Center-
Autor(es): dc.creatorAlves-Martin, Maria Fernanda-
Autor(es): dc.creatorBertozzo, Thainá Valente-
Autor(es): dc.creatorAires, Isabella Neves-
Autor(es): dc.creatorManzini, Suzane-
Autor(es): dc.creatorPaixão-Marques, Mirian dos Santos-
Autor(es): dc.creatorGuiraldi, Lívia Maísa-
Autor(es): dc.creatordos Santos, Wesley José-
Autor(es): dc.creatorSánchez, Gabriela Pacheco-
Autor(es): dc.creatorCurci, Vera Cláudia Lorenzetti Magalhães-
Autor(es): dc.creatorRichini-Pereira, Virgínia Bodelão-
Autor(es): dc.creatorLucheis, Simone Baldini-
Data de aceite: dc.date.accessioned2025-08-21T19:42:44Z-
Data de disponibilização: dc.date.available2025-08-21T19:42:44Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/ani13152468-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/303860-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/303860-
Descrição: dc.descriptionBackground and objectives: Feline leishmaniasis (FeL) is caused by several species of parasites of the genus Leishmania. The disease can occur with the presence or absence of clinical signs, similar to those observed in other common infectious diseases. In endemic regions for FeL, the infection has been associated with dermatological lesions. Therefore, considering the search for less invasive and more effective diagnostic techniques, we aimed to investigate the presence of Leishmania spp. in domestic cats through Polymerase Chain Reaction (PCR) and high-resolution melting (HRM) analyses of conjunctival, oral, and nasal epithelial cells, and we detected the presence of anti-Leishmania IgG antibodies from serological techniques of the Immunofluorescent Antibody Test (IFAT) and ELISA. Methods: The PCR and HRM for detection of Leishmania spp. were performed on 36 samples of epithelial cells from the conjunctiva of male and female cats, collected using sterile swabs. The serological tests IFAT and ELISA were also performed. Results: The prevalence of Leishmania donovani infection was 11.1% (4/36) by PCR assay, and those results were confirmed for Leishmania species using the HRM technique. Twenty-four cats (24/36 = 66.7%) were reactive to the IFAT and twenty-two cats were reactive by the ELISA technique (22/36 = 61.1%). Interpretation and Conclusions: The use of conjunctival swabs was shown to be a non-invasive, practical, and easy-to-perform technique, and in addition to the genetic sequencing and HRM, it was able to identify the parasitic DNA of L. donovani in cats. This technique can be used for screening diagnosis in future epidemiological surveys of FeL and can be used as a complement to clinical and/or serological tests, as well as associating the clinical history of the animal, for the diagnostic conclusion.-
Descrição: dc.descriptionDepartment of Biology and Animal Science School of Engineering Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Tropical Diseases Botucatu Medical School Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Molecular Biology and Biochemistry School of Biological Sciences University of California Irvine (UCI)-
Descrição: dc.descriptionBiological Institute Araçatuba Regional Laboratory, SP-
Descrição: dc.descriptionAdolfo Lutz Institute Bauru II Regional Laboratory Center, SP-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Biology and Animal Science School of Engineering Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Tropical Diseases Botucatu Medical School Sao Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Sciences Sao Paulo State University (UNESP), SP-
Idioma: dc.languageen-
Relação: dc.relationAnimals-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectcats-
Palavras-chave: dc.subjectconjunctival swabs-
Palavras-chave: dc.subjectfeline leishmaniasis-
Palavras-chave: dc.subjectHRM-
Palavras-chave: dc.subjectPCR-
Título: dc.titleDetection of Leishmania spp. in Cats: Analysis of Nasal, Oral and Conjunctival Swabs by PCR and HRM-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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