Alginate-Based 3D A549 Cell Culture Model to Study Paracoccidioides Infection

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorCentro Universitário Central Paulista—UNICEP-
Autor(es): dc.creatordos Santos, Kelvin Sousa-
Autor(es): dc.creatorOliveira, Lariane Teodoro-
Autor(es): dc.creatorde Lima Fontes, Marina-
Autor(es): dc.creatorMigliato, Ketylin Fernanda-
Autor(es): dc.creatorFusco-Almeida, Ana Marisa-
Autor(es): dc.creatorMendes Giannini, Maria José Soares-
Autor(es): dc.creatorMoroz, Andrei-
Data de aceite: dc.date.accessioned2025-08-21T15:27:12Z-
Data de disponibilização: dc.date.available2025-08-21T15:27:12Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/jof9060634-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/303515-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/303515-
Descrição: dc.descriptionA three-dimensional (3D) lung aggregate model based on sodium alginate scaffolds was developed to study the interactions between Paracoccidioides brasiliensis (Pb) and lung epithelial cells. The suitability of the 3D aggregate as an infection model was examined using cell viability (cytotoxicity), metabolic activity, and proliferation assays. Several studies exemplify the similarity between 3D cell cultures and living organisms, which can generate complementary data due to the greater complexity observed in these designed models, compared to 2D cell cultures. A 3D cell culture system of human A549 lung cell line plus sodium alginate was used to create the scaffolds that were infected with Pb18. Our results showed low cytotoxicity, evidence of increased cell density (indicative of cell proliferation), and the maintenance of cell viability for seven days. The confocal analysis revealed viable yeast within the 3D scaffold, as demonstrated in the solid BHI Agar medium cultivation. Moreover, when ECM proteins were added to the alginate scaffolds, the number of retrieved fungi was significantly higher. Our results highlight that this 3D model may be promising for in vitro studies of host–pathogen interactions.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionDepartment of Clinical Analyses School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionCentro Universitário Central Paulista—UNICEP, São Paulo-
Descrição: dc.descriptionDepartment of Clinical Analyses School of Pharmaceutical Sciences São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionCAPES: 134011/2018-0-
Idioma: dc.languageen-
Relação: dc.relationJournal of Fungi-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subject3D culture-
Palavras-chave: dc.subjectfungi infection-
Palavras-chave: dc.subjectscaffolds-
Palavras-chave: dc.subjectsodium alginate-
Título: dc.titleAlginate-Based 3D A549 Cell Culture Model to Study Paracoccidioides Infection-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

Não existem arquivos associados a este item.