Engineering resveratrol-loaded chitosan nanoparticles for potential use against Helicobacter pylori infection

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorRua Alfredo Allen-
Autor(es): dc.creatorSpósito, Larissa-
Autor(es): dc.creatorFonseca, Diana-
Autor(es): dc.creatorGonçalves Carvalho, Suzana-
Autor(es): dc.creatorSábio, Rafael Miguel-
Autor(es): dc.creatorMarena, Gabriel Davi-
Autor(es): dc.creatorBauab, Taís Maria-
Autor(es): dc.creatorBagliotti Meneguin, Andréia-
Autor(es): dc.creatorParreira, Paula-
Autor(es): dc.creatorL. Martins, M. Cristina-
Autor(es): dc.creatorChorilli, Marlus-
Data de aceite: dc.date.accessioned2025-08-21T20:00:31Z-
Data de disponibilização: dc.date.available2025-08-21T20:00:31Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-06-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.ejpb.2024.114280-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/303083-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/303083-
Descrição: dc.descriptionHelicobacter pylori (H. pylori) is a microorganism directly linked to severe clinical conditions affecting the stomach. The virulence factors and its ability to form biofilms increase resistance to conventional antibiotics, growing the need for new substances and strategies for the treatment of H. pylori infection. The trans-resveratrol (RESV), a bioactive polyphenol from natural sources, has a potential activity against this gastric pathogen. Here, Chitosan nanoparticles (NP) containing RESV (RESV-NP) were developed for H. pylori management. The RESV-NP were prepared using the ionic gelation method and characterized by Dynamic Light Scattering (DLS), Nanoparticle Tracking Analysis (NTA) and, Cryogenic Transmission Electron Microscopy (Cryo – TEM). The encapsulation efficiency (EE) and in vitro release rate of RESV were quantified using high-performance liquid chromatography (HPLC). RESV-NP performance against H. pylori was evaluated by the quantification of the minimum inhibitory/bactericidal concentrations (MIC/MBC), time to kill, alterations in H. pylori morphology in its planktonic form, effects against H. pylori biofilm and in an in vitro infection model. RESV-NP cytotoxicity was evaluated against AGS and MKN-74 cell lines and by hemolysis assay. Acute toxicity was tested using Galleria mellonella model assays. RESV-NP showed a spherical shape, size of 145.3 ± 24.7 nm, polydispersity index (PDI) of 0.28 ± 0.008, and zeta potential (ZP) of + 16.9 ± 1.81 mV in DLS, while particle concentration was 3.12 x 1011 NP/mL (NTA). RESV-NP EE was 72 %, with full release within the first 5 min. In microbiological assays, RESV-NP presented a MIC/MBC of 3.9 µg/mL, a time to kill of 24 h for complete eradication of H. pylori. At a concentration of 2xMIC (7.8 µg/mL), RESV-NP completely eradicated the H. pylori biofilm, and in an in vitro infection model, RESV-NP (4xMIC – 15.6 µg/mL) showed a significant decrease in bacterial load (1 Log10CFU/mL) when compared to the H. pylori J99 control. In addition, they did not demonstrate a toxic character at MIC concentration for both cell lines. The use of the RESV-NP with mucoadhesion profile is an interesting strategy for oral administration of substances targeting gastric disorders, linked to H. pylori infections.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Biological Sciences, SP-
Descrição: dc.descriptioni3S-Instituto de Investigação e Inovação em Saúde Rua Alfredo Allen, 208-
Descrição: dc.descriptionINEB-Instituto de Engenharia Biomédica Rua Alfredo Allen, 208-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines, SP-
Descrição: dc.descriptionSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Biological Sciences, SP-
Idioma: dc.languageen-
Relação: dc.relationEuropean Journal of Pharmaceutics and Biopharmaceutics-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntibacterial activity-
Palavras-chave: dc.subjectDrug delivery system-
Palavras-chave: dc.subjectGastric infection-
Palavras-chave: dc.subjectHelicobacter pylori-
Palavras-chave: dc.subjectPolymeric nanoparticles-
Palavras-chave: dc.subjectTrans-resveratrol-
Título: dc.titleEngineering resveratrol-loaded chitosan nanoparticles for potential use against Helicobacter pylori infection-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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