Recombinant sugarcane cystatin CaneCPI-5 promotes osteogenic differentiation

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.contributorUniversidade Federal de São Carlos (UFSCar)-
Autor(es): dc.creatorFernandes, Célio Junior da Costa-
Autor(es): dc.creatorCassiano, Ana Flávia Balestrero-
Autor(es): dc.creatorHenrique-Silva, Flavio-
Autor(es): dc.creatorCirelli, Joni Augusto-
Autor(es): dc.creatorde Souza, Eduardo Pereira-
Autor(es): dc.creatorCoaguila-Llerena, Hernán-
Autor(es): dc.creatorZambuzzi, Willian Fernando-
Autor(es): dc.creatorFaria, Gisele-
Data de aceite: dc.date.accessioned2025-08-21T18:49:28Z-
Data de disponibilização: dc.date.available2025-08-21T18:49:28Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-08-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.tice.2023.102157-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/302780-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/302780-
Descrição: dc.descriptionCysteine proteases orchestrate bone remodeling, and are inhibited by cystatins. In reinforcing our hypothesis that exogenous and naturally obtained inhibitors of cysteine proteases (cystatins) act on bone remodeling, we decided to challenge osteoblasts with sugarcane-derived cystatin (CaneCPI-5) for up to 7 days. To this end, we investigated molecular issues related to the decisive, preliminary stages of osteoblast biology, such as adhesion, migration, proliferation, and differentiation. Our data showed that CaneCPI-5 negatively modulates both cofilin phosphorylation at Ser03, and the increase in cytoskeleton remodeling during the adhesion mechanism, possibly as a prerequisite to controlling cell proliferation and migration. This is mainly because CaneCPI-5 also caused the overexpression of the CDK2 gene, and greater migration of osteoblasts. Extracellular matrix remodeling was also evaluated in this study by investigating matrix metalloproteinase (MMP) activities. Our data showed that CaneCPI-5 overstimulates both MMP-2 and MMP-9 activities, and suggested that this cellular event could be related to osteoblast differentiation. Additionally, differentiation mechanisms were better evaluated by investigating Osterix and alkaline phosphatase (ALP) genes, and bone morphogenetic protein (BMP) signaling members. Altogether, our data showed that CaneCPI-5 can trigger biological mechanisms related to osteoblast differentiation, and broaden the perspectives for better exploring biotechnological approaches for bone disorders.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionBioassays and Cell Dynamics Lab Department of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University – UNESP, São Paulo-
Descrição: dc.descriptionExercise Cell Biology Lab School of Applied Sciences University of Campinas, São Paulo-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences Sao Paulo State University – UNESP, São Paulo-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry at Araraquara Sao Paulo State University – UNESP, São Paulo-
Descrição: dc.descriptionDepartment of Genetics and Evolution Federal University of Sao Carlos, São Paulo-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University –UNESP, São Paulo-
Descrição: dc.descriptionBioassays and Cell Dynamics Lab Department of Chemical and Biological Sciences Institute of Biosciences Sao Paulo State University – UNESP, São Paulo-
Descrição: dc.descriptionDepartment of Biophysics and Pharmacology Institute of Biosciences Sao Paulo State University – UNESP, São Paulo-
Descrição: dc.descriptionDepartment of Restorative Dentistry School of Dentistry at Araraquara Sao Paulo State University – UNESP, São Paulo-
Descrição: dc.descriptionDepartment of Diagnosis and Surgery School of Dentistry at Araraquara Sao Paulo State University –UNESP, São Paulo-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2016/08888-9, 2017/05784-0, 2018/24662-6, 2019/21807-6-
Descrição: dc.descriptionFAPESP: 2020/09576-6-
Descrição: dc.descriptionCNPq: 311746/2017-9-
Idioma: dc.languageen-
Relação: dc.relationTissue and Cell-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectBone-
Palavras-chave: dc.subjectCathepsin-
Palavras-chave: dc.subjectCell differentiation-
Palavras-chave: dc.subjectOsteoblasts-
Palavras-chave: dc.subjectPhytocystatin-
Título: dc.titleRecombinant sugarcane cystatin CaneCPI-5 promotes osteogenic differentiation-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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