Synergistic Antibacterial Efficacy of Melittin in Combination with Oxacillin against Methicillin-Resistant Staphylococcus aureus (MRSA)

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Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorPereira, Ana Flávia Marques-
Autor(es): dc.creatorSani, Alessandra Aguirra-
Autor(es): dc.creatorZapata, Tatiane Baptista-
Autor(es): dc.creatorSousa, Débora Silva Marques de-
Autor(es): dc.creatorRossini, Bruno César-
Autor(es): dc.creatorSantos, Lucilene Delazari dos-
Autor(es): dc.creatorRall, Vera Lúcia Mores-
Autor(es): dc.creatorRiccardi, Carla dos Santos-
Autor(es): dc.creatorFernandes Júnior, Ary-
Data de aceite: dc.date.accessioned2025-08-21T17:36:22Z-
Data de disponibilização: dc.date.available2025-08-21T17:36:22Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-11-30-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/microorganisms11122868-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/302666-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/302666-
Descrição: dc.descriptionMethicillin-resistant Staphylococcus aureus (MRSA) often cause infections with high mortality rates. Antimicrobial peptides are a source of molecules for developing antimicrobials; one such peptide is melittin, a fraction from the venom of the Apis mellifera bee. This study aimed to evaluate the antibacterial and antibiofilm activities of melittin and its association with oxacillin (mel+oxa) against MRSA isolates, and to investigate the mechanisms of action of the treatments on MRSA. Minimum inhibitory concentrations (MICs) were determined, and synergistic effects of melittin with oxacillin and cephalothin were assessed. Antibiofilm and cytotoxic activities, as well as their impact on the cell membrane, were evaluated for melittin, oxacillin, and mel+oxa. Proteomics evaluated the effects of the treatments on MRSA. Melittin mean MICs for MRSA was 4.7 μg/mL and 12 μg/mL for oxacillin. Mel+oxa exhibited synergistic effects, reducing biofilm formation, and causing leakage of proteins, nucleic acids, potassium, and phosphate ions, indicating action on cell membrane. Melittin and mel+oxa, at MIC values, did not induce hemolysis and apoptosis in HaCaT cells. The treatments resulted in differential expression of proteins associated with protein synthesis and energy metabolism. Mel+oxa demonstrated antibacterial activity against MRSA, suggesting a potential as a candidate for the development of new antibacterial agents against MRSA.-
Descrição: dc.descriptionThe Center for the Study of Venoms and Venomous Animals of UNESP (CEVAP) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Microbiology and Immunology Sector Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP), Botucatu 18618-689-
Descrição: dc.descriptionInstitute of Biotechnology (IBTEC) São Paulo State University (UNESP), Botucatu 18607-440-
Descrição: dc.descriptionGraduate Program in Tropical Diseases and Graduate Program in Research and Development (Medical Biotechnology Botucatu Medical School (FMB) São Paulo State University (UNESP), Botucatu 18618-687-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology Faculty of Agricultural Sciences (FCA) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionThe Center for the Study of Venoms and Venomous Animals of UNESP (CEVAP) São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Chemical and Biological Sciences Microbiology and Immunology Sector Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP), Botucatu 18618-689-
Descrição: dc.descriptionInstitute of Biotechnology (IBTEC) São Paulo State University (UNESP), Botucatu 18607-440-
Descrição: dc.descriptionGraduate Program in Tropical Diseases and Graduate Program in Research and Development (Medical Biotechnology Botucatu Medical School (FMB) São Paulo State University (UNESP), Botucatu 18618-687-
Descrição: dc.descriptionDepartment of Bioprocesses and Biotechnology Faculty of Agricultural Sciences (FCA) São Paulo State University (UNESP), São Paulo-
Idioma: dc.languageen-
Relação: dc.relationMicroorganisms-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectantibiofilm activity-
Palavras-chave: dc.subjectantibiotic resistance-
Palavras-chave: dc.subjectantimicrobial peptides-
Palavras-chave: dc.subjectbiofilm-
Palavras-chave: dc.subjectproteomics-
Palavras-chave: dc.subjectsynergism-
Título: dc.titleSynergistic Antibacterial Efficacy of Melittin in Combination with Oxacillin against Methicillin-Resistant Staphylococcus aureus (MRSA)-
Tipo de arquivo: dc.typelivro digital-
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