Serial frequent or multiple Tru-cut® testicular biopsies in rams enable assessment of histological characteristics or transcriptional profiles, with no acute or chronic adverse effects

Registro completo de metadados
MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorSanto Amaro University-
Autor(es): dc.contributorGhent University-
Autor(es): dc.contributorUniversidade Estadual de Londrina (UEL)-
Autor(es): dc.contributorUniversity of Western São Paulo (Unoeste)-
Autor(es): dc.contributorUniversity of Calgary-
Autor(es): dc.creatorPupulim, A. G.R.-
Autor(es): dc.creatorRattes, P. Z.-
Autor(es): dc.creatorMogollón García, H. D.-
Autor(es): dc.creatorCarvalho, J. C.-
Autor(es): dc.creatorUzae, K. Z.-
Autor(es): dc.creatorRibeiro, G. C.-
Autor(es): dc.creatorRizzoto, G.-
Autor(es): dc.creatorDenadai, R.-
Autor(es): dc.creatorNogueira, G. P.-
Autor(es): dc.creatorNavolar, F. M.N.-
Autor(es): dc.creatorDi Santis, G. W.-
Autor(es): dc.creatorNunes, S. G.-
Autor(es): dc.creatorCastilho, A. C.S.-
Autor(es): dc.creatorKastelic, J. P.-
Autor(es): dc.creatorFerreira, J. C.P.-
Data de aceite: dc.date.accessioned2025-08-21T19:42:22Z-
Data de disponibilização: dc.date.available2025-08-21T19:42:22Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-12-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2024.10.007-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/302659-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/302659-
Descrição: dc.descriptionThis study aimed to evaluate the feasibility of performing multiple testicular biopsies in rams using Tru-cut® needles, assessing histological structure, gene expression, and potential complications such as effects on semen quality, testicular blood flow, and ultrasonographic echotexture. In Exp. 1, six mature rams underwent testicular biopsies at intervals (0, 3, 6, 12, 24, and 48 h) using a 16 G Tru-cut® needle, with alternating testes for each collection. Benzathine benzylpenicillin and flunixin meglumine were administered for infection and inflammation control. Local anesthesia and post-biopsy care included lidocaine, digital pressure, and ice application. Testicular samples were analyzed for gene expression related to inflammation, oxidative stress, and steroidogenesis. Semen quality was assessed pre-biopsy and 28 days post-biopsy. Ultrasonographic evaluations of the scrotum and testes were conducted before biopsies and on days 5, 9, 13, 17, and 21 post-biopsies. In Exp. 2, a second group of six mature rams underwent biopsies using 14 G needles, with two samples taken from each testis. Samples were histologically examined for structural preservation. Scrotal skin temperature was measured using infrared thermography, and testicular blood flow was assessed via color Doppler ultrasonography, with measurements taken before and on days 1, 2, 4, 6, 8, 10, 25, 50, 75, and 100 post-biopsies. Semen collection followed the same schedule as in Exp. 1. In Exp. 3, biopsies were performed on different testicular regions (upper, middle, lower) using 12 G, 14 G, and 16 G needles to compare structural preservation. Samples were histologically analyzed. No clinical signs of injury, inflammation, or fluid accumulation were observed. Scrotal pain, increased temperature, swelling, and bleeding were absent, and behavioral signs indicative of pain were not detected. Gene expression remained unchanged, and no significant alterations in seminal characteristics or testicular echogenicity were observed. A slight increase in resistivity and pulsatility indices was noted in Exp. 2. Biopsies with 14 G and 16 G needles resulted in structural disruptions, while 12 G needles better preserved testicular parenchyma. Multiple testicular biopsies using Tru-cut® needles did not cause significant morphological changes, alter transcriptional profiles, or affect semen or ultrasonographic characteristics, demonstrating that this method is viable for monitoring acute molecular changes in the testes.-
Descrição: dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Clinical and Toxicological Analysis University of São Paulo (USP), SP-
Descrição: dc.descriptionSanto Amaro University, SP-
Descrição: dc.descriptionDepartment of Veterinary Clinical Science School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Internal Medicine Reproduction and Population Medicine Faculty of Veterinary Sciences Ghent University, EF-
Descrição: dc.descriptionDepartment of Animal Health and Production FMVA Sao Paulo State University UNESP, SP-
Descrição: dc.descriptionDepartment of Preventive Veterinary Medicine Londrina State University, PR-
Descrição: dc.descriptionDepartment of Pharmacology Bioscience Institute São Paulo State University (UNESP), SP-
Descrição: dc.descriptionUniversity of Western São Paulo (Unoeste), SP-
Descrição: dc.descriptionFaculty of Veterinary Medicine University of Calgary-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Veterinary Clinical Science School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SP-
Descrição: dc.descriptionDepartment of Animal Health and Production FMVA Sao Paulo State University UNESP, SP-
Descrição: dc.descriptionDepartment of Pharmacology Bioscience Institute São Paulo State University (UNESP), SP-
Descrição: dc.descriptionCAPES: 001-
Descrição: dc.descriptionFAPESP: 2020/15556–8-
Descrição: dc.descriptionCNPq: 3845-
Formato: dc.format81-89-
Idioma: dc.languageen-
Relação: dc.relationTheriogenology-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAndrological evaluation-
Palavras-chave: dc.subjectBiopsy-
Palavras-chave: dc.subjectGene expression-
Palavras-chave: dc.subjectSemen-
Palavras-chave: dc.subjectSperm-
Palavras-chave: dc.subjectTestis-
Título: dc.titleSerial frequent or multiple Tru-cut® testicular biopsies in rams enable assessment of histological characteristics or transcriptional profiles, with no acute or chronic adverse effects-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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