Immunostaining and gene expression of epidermal growth factor receptors (HER1/HER2) in canine cutaneous squamous cell carcinoma

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Federal de Goiás (UFG)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.creatorMagalhães, Paula Lima-
Autor(es): dc.creatorNascente, Eduardo de Paula-
Autor(es): dc.creatorFaleiro, Mariana Batista Rodrigues-
Autor(es): dc.creatorAlmeida, Ana Maria de Souza-
Autor(es): dc.creatorAlves, Carlos Eduardo Fonseca-
Autor(es): dc.creatorde Moura, Veridiana Maria Brianezi Dignani-
Data de aceite: dc.date.accessioned2025-08-21T15:57:44Z-
Data de disponibilização: dc.date.available2025-08-21T15:57:44Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-09-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.rvsc.2024.105363-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/302427-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/302427-
Descrição: dc.descriptionCutaneous squamous cell carcinoma (cSCC) is a neoplasm type often diagnosed in dogs. However, studies focused on further investigating its molecular biology, mainly biomarkers to help implementing new therapies, remain scare in the literature. Thus, immunostaining and the gene expression of epidermal growth factor receptors (HER1 and HER2) in canine cSCC presenting different cell differentiation degrees were herein assessed. Thirty-two (32) canine cSCC were selected, classified based on to their cell differentiation degree and subjected to immunohistochemical study to assess HER1 and HER2 immunostaining intensity and distribution. In addition, HER1 and HER2 gene expression was investigated through real-time PCR. Membranous and cytoplasmic immunostaining were observed in both markers. HER2 prevailed in poorly differentiated cSCC; there was positive protein expression correlation between both markers. Mean HER1 gene expression was higher in moderately differentiated, whereas mean HER2 gene expression was higher in poorly differentiated cSCC. Moreover, there was gene expression correlation between markers, regardless of cell differentiation degree. Thus, HER2 protein immunostaining and gene expression were higher in poorly differentiated canine cSCC and it enabled understanding that increase observed in this epidermal growth factor receptor is proportional to this neoplasm's cell differentiation degree in canine species. Results in the current study helped better understanding canine cSCC's molecular biology; however, it is relevant studying other markers aiming to investigate signaling pathways.-
Descrição: dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)-
Descrição: dc.descriptionDepartment of Veterinary Medicine School of Veterinary Medicine and Animal Science Federal University of Goiás, Goiás-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), São Paulo-
Descrição: dc.descriptionDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP), São Paulo-
Idioma: dc.languageen-
Relação: dc.relationResearch in Veterinary Science-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectEGFR-
Palavras-chave: dc.subjectImmunohistochemistry-
Palavras-chave: dc.subjectReal-time PCR-
Palavras-chave: dc.subjectSkin-
Palavras-chave: dc.subjectTyrosine kinase-
Título: dc.titleImmunostaining and gene expression of epidermal growth factor receptors (HER1/HER2) in canine cutaneous squamous cell carcinoma-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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