Recombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response

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MetadadosDescriçãoIdioma
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorUniversidade Estadual de Campinas (UNICAMP)-
Autor(es): dc.creatorAssugeni, Isabela Oliveira Sandrini-
Autor(es): dc.creatorBazon, Murilo Luiz-
Autor(es): dc.creatorPinto, Lucas Machado-
Autor(es): dc.creatorMainente, Leonardo Antônio Benedito-
Autor(es): dc.creatorBrochetto-Braga, Márcia Regina-
Autor(es): dc.creatorde Lima Zollner, Ricardo-
Autor(es): dc.creatorFernandes, Luís Gustavo Romani-
Data de aceite: dc.date.accessioned2025-08-21T20:26:32Z-
Data de disponibilização: dc.date.available2025-08-21T20:26:32Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2023-10-31-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.1016/j.jim.2023.113557-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/302321-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/302321-
Descrição: dc.descriptionPolybia paulista is a neotropical social wasp related to severe accidents and allergic reactions cases, including anaphylaxis, in southeastern Brazil. Antigen 5 (Poly p 5) is a major allergenic protein from its venom with potential use for component-resolved diagnostic. Therefore, the previous characterization of the immune response profile triggered by Poly p 5 should be evaluated. Recombinant Poly p 5 (rPoly p 5) was used to sensitize BALB/c mice with six weekly intradermal doses, and the specific antibody production and the functional profile of CD4+ T cells were assessed. rPoly p 5 induced the production of specific immunoglobulins (sIg) sIgE, sIgG1 and sIgG2a, which could recognize natural Poly p 5 presented in the venom of four different wasp species. rPoly p 5 stimulated in vitro the CD4+ T cells from immunized mice, which showed a significant proliferative response. These antigen-specific CD4+T cells produced IFN-γ and IL-17A cytokines and increased ROR-γT transcription factor expression. No differences between the control group and sensitized mice were found in IL-4 production and GATA-3 and T-bet expression. Interestingly, increased CD25+FoxP3+ regulatory T cells (Tregs) frequency was observed in the splenocyte cell cultures from rPoly p 5 immunized mice after the in vitro stimulation with both P. paulista venom extract and rPoly p 5. Here we showed that rPoly p 5 induces antigen-specific antibodies capable of recognizing Antigen 5 in the venom of four wasp species and modulates antigen-specific CD4+ T cells to IFN-γ production response associated with a Th17 profile in sensitized mice. These findings emphasize the potential use of rPoly p 5 as an essential source of a major wasp allergen with significant immunological properties.-
Descrição: dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)-
Descrição: dc.descriptionLaboratory of Arthropods Molecular Biology (LBMA) Institute of Biosciences of Rio Claro Department of Biology São Paulo State University, SP-
Descrição: dc.descriptionLaboratory of Translational Immunology (LIT) School of Medical Sciences University of Campinas (UNICAMP), SP-
Descrição: dc.descriptionLaboratory of Arthropods Molecular Biology (LBMA) Institute of Biosciences of Rio Claro Department of Biology São Paulo State University, SP-
Descrição: dc.descriptionFAPESP: 2017/07988-2-
Descrição: dc.descriptionFAPESP: 2019/00729-7-
Descrição: dc.descriptionFAPESP: 2019/22024-5-
Idioma: dc.languageen-
Relação: dc.relationJournal of Immunological Methods-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectAntigen 5-
Palavras-chave: dc.subjectCD4+ T cells-
Palavras-chave: dc.subjectCytokines production-
Palavras-chave: dc.subjectHymenoptera venom allergen-
Palavras-chave: dc.subjectPolybia paulista-
Palavras-chave: dc.subjectSpecific immunoglobulin-
Título: dc.titleRecombinant antigen 5 from Polybia paulista wasp venom (Hymenoptera, Vespidae): Antigen-specific antibody production and functional profile of CD4+ T cells in the immune response-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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