EMC1 Is Required for the Sarcoplasmic Reticulum and Mitochondrial Functions in the Drosophila Muscle

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Autor(es): dc.contributorUniversidade de São Paulo (USP)-
Autor(es): dc.contributorUniversidade Estadual Paulista (UNESP)-
Autor(es): dc.contributorFederal University of Alagoas-
Autor(es): dc.contributorThe City University of New York-
Autor(es): dc.creatorCouto-Lima, Carlos Antonio-
Autor(es): dc.creatorMachado, Maiaro Cabral Rosa-
Autor(es): dc.creatorAnhezini, Lucas-
Autor(es): dc.creatorOliveira, Marcos Túlio-
Autor(es): dc.creatorMolina, Roberto Augusto da Silva-
Autor(es): dc.creatorda Silva, Rodrigo Ribeiro-
Autor(es): dc.creatorLopes, Gabriel Sarti-
Autor(es): dc.creatorTrinca, Vitor-
Autor(es): dc.creatorColón, David Fernando-
Autor(es): dc.creatorPeixoto, Pablo M.-
Autor(es): dc.creatorMonesi, Nadia-
Autor(es): dc.creatorAlberici, Luciane Carla-
Autor(es): dc.creatorRamos, Ricardo Guelerman P.-
Autor(es): dc.creatorEspreafico, Enilza Maria-
Data de aceite: dc.date.accessioned2025-08-21T17:15:35Z-
Data de disponibilização: dc.date.available2025-08-21T17:15:35Z-
Data de envio: dc.date.issued2025-04-29-
Data de envio: dc.date.issued2024-10-01-
Fonte completa do material: dc.identifierhttp://dx.doi.org/10.3390/biom14101258-
Fonte completa do material: dc.identifierhttps://hdl.handle.net/11449/302179-
Fonte: dc.identifier.urihttp://educapes.capes.gov.br/handle/11449/302179-
Descrição: dc.descriptionEMC1 is part of the endoplasmic reticulum (ER) membrane protein complex, whose functions include the insertion of transmembrane proteins into the ER membrane, ER–mitochondria contact, and lipid exchange. Here, we show that the Drosophila melanogaster EMC1 gene is expressed in the somatic musculature and the protein localizes to the sarcoplasmic reticulum (SR) network. Muscle-specific EMC1 RNAi led to severe motility defects and partial late pupae/early adulthood lethality, phenotypes that are rescued by co-expression with an EMC1 transgene. Motility impairment in EMC1-depleted flies was associated with aberrations in muscle morphology in embryos, larvae, and adults, including tortuous and misaligned fibers with reduced size and weakness. They were also associated with an altered SR network, cytosolic calcium overload, and mitochondrial dysfunction and dysmorphology that impaired membrane potential and oxidative phosphorylation capacity. Genes coding for ER stress sensors, mitochondrial biogenesis/dynamics, and other EMC components showed altered expression and were mostly rescued by the EMC1 transgene expression. In conclusion, EMC1 is required for the SR network’s mitochondrial integrity and influences underlying programs involved in the regulation of muscle mass and shape. We believe our data can contribute to the biology of human diseases caused by EMC1 mutations.-
Descrição: dc.descriptionDepartment of Cell and Molecular Biology Faculty of Medicine of Ribeirão Preto University of São Paulo (FMRP-USP), SP-
Descrição: dc.descriptionDepartment of Biotechnology College of Agricultural and Veterinary Sciences Sao Paulo State University, SP-
Descrição: dc.descriptionCellular and Molecular Biology Program Faculty of Medicine of Ribeirão Preto University of São Paulo (FMRP-USP), SP-
Descrição: dc.descriptionInstitute of Biological Sciences and Health Federal University of Alagoas, AL-
Descrição: dc.descriptionDepartment of Pharmacology Ribeirão Preto Medical School University of São Paulo, Ribeirão Preto, SP-
Descrição: dc.descriptionBaruch College and Graduate Center The City University of New York-
Descrição: dc.descriptionDepartment of Clinical Analyses Toxicology and Food Science School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, Ribeirão Preto, SP-
Descrição: dc.descriptionDepartment of Biomolecular Sciences School of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, Ribeirão Preto, SP-
Descrição: dc.descriptionDepartment of Biotechnology College of Agricultural and Veterinary Sciences Sao Paulo State University, SP-
Idioma: dc.languageen-
Relação: dc.relationBiomolecules-
???dc.source???: dc.sourceScopus-
Palavras-chave: dc.subjectendoplasmic reticulum membrane protein complex-
Palavras-chave: dc.subjectmitochondria-
Palavras-chave: dc.subjectmusculature-
Título: dc.titleEMC1 Is Required for the Sarcoplasmic Reticulum and Mitochondrial Functions in the Drosophila Muscle-
Tipo de arquivo: dc.typelivro digital-
Aparece nas coleções:Repositório Institucional - Unesp

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